Human NAD(P)H dehydrogenase [quinone] 1 ELISA kit (E01N0670)

BlueGene Biotech Human NAD(P)H dehydrogenase [quinone] 1 ELISA kit (E01N0670)

E01N0670 Human NAD(P)H dehydrogenase [quinone] 1 ELISA kit

The Human NAD(P)H dehydrogenase [quinone] 1 ELISA kit can be used to identify samples from the human species. NAD(P)H dehydrogenase [quinone] 1 can also be called NQO1, DHQU, DIA4, DTD, NMOR1, NMORI, QR1, Azoreductase, DT-diaphorase, Menadione reductase, Phylloquinone reductase, Quinone reductase 1, NAD(P)H:quinone oxidoreductase 1, DT-diaphorase 1 Publication.

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Specifications of Human NAD(P)H dehydrogenase [quinone] 1 ELISA kit

Product Information
Cat. No.	E01N0670
Product Name	Human NAD(P)H dehydrogenase [quinone] 1 ELISA kit
Species	Human
Product Size	48 Tests / 96 Tests
Concentration	1.0-25 ng/ml
Sensitivity	0.1 ng/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	1.0 ng/mL	1 vial
STANDARD C (0.5mL)	2.5 ng/mL	1 vial
STANDARD D (0.5mL)	5.0 ng/mL	1 vial
STANDARD E (0.5mL)	10 ng/mL	1 vial
STANDARD F (0.5mL)	25 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

NQO1 ELISA kit uses an anti-NQO1 antibody and an NQO1-HRP conjugate in a competitive enzyme immunoassay method. NQO1-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-NQO1 antibody binding site between NQO1 from samples and NQO1-HRP conjugate, the intensity of the color is inversely proportional to the concentration of NQO1. Since the number of sites is limited, as more sites are occupied by NQO1 from the sample, fewer sites are left to bind NQO1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NQO1 concentration in each sample is interpolated from this standard curve.

Quality Control on Human NAD(P)H dehydrogenase [quinone] 1 ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between NQO1 and analogues was observed.

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Summary of the Assay Procedure for Human NAD(P)H dehydrogenase [quinone] 1 ELISA kit

Citations of Human NAD(P)H dehydrogenase [quinone] 1 ELISA kit

E01N0670 has been referenced in the below publications：

To investigate the association between distribution of body fat and coronary heart disease(CHD)by analysing the correlation between body fat distribution measures and incidence，type and severity of coronary heart disease．

Pilot study of bevacizumab in combination with docetaxel and cyclophosphamide as adjuvant treatment for patients with early stage HER-2 negative breast cancer, including analysis of candidate circulating markers of cardiac toxicity: ICORG 08-10 trial.