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  • bovine serum albumin elisa 1

BlueGene Biotech Human Neutrophil Elastase ELISA kit (E01N0006)

E01N0006 Human Neutrophil Elastase ELISA kit

The Human Neutrophil Elastase ELISA kit can be used to identify samples from the human species. Neutrophil Elastase can also be called ELANE, HLE, HNE, ELA2, PMN-E, Neutrophil Elastase, Medullasin, Polymorphonuclear Leukocyte Elastase, Bone Marrow Serine Protease, Human Leukocyte Elastase, PMN Elastase.

Products

Specifications of Human Neutrophil Elastase ELISA kit

Product Information

Cat. No.

E01N0006

Product Name

Human Neutrophil Elastase ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

2.5-50 ng/mL

Sensitivity

0.1 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

2.5 ng/mL

1 vial

STANDARD C (0.5mL)

5.0 ng/mL

1 vial

STANDARD D (0.5mL)

10 ng/mL

1 vial

STANDARD E (0.5mL)

25 ng/mL

1 vial

STANDARD F (0.5mL)

50 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

NE ELISA kit uses an anti-NE antibody and an NE-HRP conjugate in a competitive enzyme immunoassay method. NE-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-NE antibody binding site between NE from samples and NE-HRP conjugate, the intensity of the color is inversely proportional to the concentration of NE. Since the number of sites is limited, as more sites are occupied by NE from the sample, fewer sites are left to bind NE-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NE concentration in each sample is interpolated from this standard curve.


Quality Control on Human Neutrophil Elastase ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between NE and analogues was observed.


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Summary of the Assay Procedure for Human Neutrophil Elastase ELISA kit

Summary of the Assay Procedure for Human Neutrophil Elastase ELISA kit

Citations of Human Neutrophil Elastase ELISA kit

E01N0006 has been referenced in the below publications:

Effect of Prunella vulgaris Decoction combined with Nifedipine in the treatment of primary hypertension.

Effect of Balanced Ultrafiltration on Plasma Concentration of Antimicrobial Agents and Inflammatory Factors during Cardiac Surgery.

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