E01N0013 Human Noradrenaline ELISA kit
The Human Noradrenaline ELISA kit can be used to identify samples from the human species. Noradrenaline can also be called NA, NAd, Norepinephrine, Norepi.
Specifications of Human Noradrenaline ELISA kit
Product Information | |
Cat. No. | E01N0013 |
Product Name | Human Noradrenaline ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 10-250 ng/dL |
Sensitivity | 1.0 ng/dL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/dL | 1 vial |
STANDARD B (0.5mL) | 10 ng/dL | 1 vial |
STANDARD C (0.5mL) | 25 ng/dL | 1 vial |
STANDARD D (0.5mL) | 50 ng/dL | 1 vial |
STANDARD E (0.5mL) | 100 ng/dL | 1 vial |
STANDARD F (0.5mL) | 250 ng/dL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
NADR ELISA kit uses an anti-NADR antibody and an NADR-HRP conjugate in a competitive enzyme immunoassay method. NADR-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-NADR antibody binding site between NADR from samples and NADR-HRP conjugate, the intensity of the color is inversely proportional to the concentration of NADR. Since the number of sites is limited, as more sites are occupied by NADR from the sample, fewer sites are left to bind NADR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NADR concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Noradrenaline ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between NADR and analogues was observed. | |
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Summary of the Assay Procedure for Human Noradrenaline ELISA kit
Citations of Human Noradrenaline ELISA kit
E01N0013 has been referenced in the below publications：
The influences of stellate ganglion block on the level of stress hormone for patients of cephalic and facial postherpetic neuralgia.
Role of remifentanil in inhibiting the stress response in gynecologic laparoscopic surgery.
Changes of ET-1 and NO in the maintenance hemodialysis complicated with intradialytic hypertension.
