E01O0022 Human Omentin 1 ELISA kit
The Human Omentin 1 ELISA kit can be used to identify samples from the human species. Omentin 1 can also be called OMT 1, HL1, INTL, ITLN, LFR, HIntL, Omentin, Intelectin 1,Galactofuranose Binding, Intestinal Lactoferrin Receptor,Endothelial lectin HL-1.
Product Information | |
Cat. No. | E01O0022 |
Product Name | Human Omentin 1 ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 250-5000 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 250 pg/mL | 1 vial |
STANDARD C (0.5mL) | 500 pg/mL | 1 vial |
STANDARD D (0.5mL) | 1000 pg/mL | 1 vial |
STANDARD E (0.5mL) | 2500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 5000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
OMT 1 ELISA kit uses an anti-OMT 1 antibody and an OMT 1-HRP conjugate in a competitive enzyme immunoassay method. OMT 1-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-OMT 1 antibody binding site between OMT 1 from samples and OMT 1-HRP conjugate, the intensity of the color is inversely proportional to the concentration of OMT 1. Since the number of sites is limited, as more sites are occupied by OMT 1 from the sample, fewer sites are left to bind OMT 1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OMT 1 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between OMT 1 and analogues was observed. | |
E01O0022 has been referenced in the below publications：
Omentin-1 is associated with carotid atherosclerosis in patients with metabolic syndrome.
Decreased Levels of Serum Omentin-1 in Patients with Inflammatory Bowel Disease.
To investigate the correlation of Omentin-1 and acute myocardial infarction (AMI).
The correlation of serum omentin-1 levels with the presence of coronary artery disease in patients with type 2 diabetes.
Clinical Analysis of Compound Dingxiangkaiwei Paste Combined Shajiganruji Treatment of 46 Cases of Children with Functional Abdominal Pain.
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