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BlueGene Biotech Human Paired box gene 9 ELISA kit (E01P0019)

  • Epigenetics and Nuclear Signaling

E01P0019 Human Paired box gene 9 ELISA kit

The Human Paired box gene 9 ELISA kit can be used to identify samples from the human species. Paired box gene 9 can also be called Paired box 9, Paired box gene 9, Paired box homeotic gene 9, Paired box protein 9, Paired box protein Pax 9, Paired box protein Pax9, Paired domain gene 9, PAX 9.

Products

Specifications of Human Paired box gene 9 ELISA kit

Product Information

Cat. No.

E01P0019

Product Name

Human Paired box gene 9 ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

100-2500 pg/ml

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

100 pg/mL

1 vial

STANDARD C (0.5mL)

250 pg/mL

1 vial

STANDARD D (0.5mL)

500 pg/mL

1 vial

STANDARD E (0.5mL)

1000 pg/mL

1 vial

STANDARD F (0.5mL)

2500 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

PAX9 ELISA kit uses an anti-PAX9 antibody and an PAX9-HRP conjugate in a competitive enzyme immunoassay method. PAX9-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-PAX9 antibody binding site between PAX9 from samples and PAX9-HRP conjugate, the intensity of the color is inversely proportional to the concentration of PAX9. Since the number of sites is limited, as more sites are occupied by PAX9 from the sample, fewer sites are left to bind PAX9-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PAX9 concentration in each sample is interpolated from this standard curve.


Quality Control on Human Paired box gene 9 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between PAX9 and analogues was observed.


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Summary of the Assay Procedure for Human Paired box gene 9 ELISA kit

Summary of the Assay Procedure for Human Paired box gene 9 ELISA kit

Citations of Human Paired box gene 9 ELISA kit

E01P0019 has been referenced in the below publications:

Expression and Clinical Significance of Pax9 in the Serum of Non-small Cell Lung Cancer Patients.

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