E01O0341 Human Oxidized lowdensity lipoprotein ELISA kit
The Human Oxidized lowdensity lipoprotein ELISA kit can be used to identify samples from the human species. Oxidized lowdensity lipoprotein can also be called Ox-LDL.
Specifications of Human Oxidized lowdensity lipoprotein ELISA kit
Product Information | |
Cat. No. | E01O0341 |
Product Name | Human Oxidized lowdensity lipoprotein ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50ng/mL |
Sensitivity | 0.1 ng/ml |
Principal | Sandwich ELISA |
Sample Volume | 50 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 10 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 10 ng/mL | 1 vial |
STANDARD E (0.5mL) | 25 ng/mL | 1 vial |
STANDARD F (0.5mL) | 50 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
OxLDL ELISA kit uses an anti-OxLDL antibody and an OxLDL-HRP conjugate in a competitive enzyme immunoassay method. OxLDL-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-OxLDL antibody binding site between OxLDL from samples and OxLDL-HRP conjugate, the intensity of the color is inversely proportional to the concentration of OxLDL. Since the number of sites is limited, as more sites are occupied by OxLDL from the sample, fewer sites are left to bind OxLDL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OxLDL concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Oxidized lowdensity lipoprotein ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between OxLDL and analogues was observed. | |
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Summary of the Assay Procedure for Human Oxidized lowdensity lipoprotein ELISA kit
Citations of Human Oxidized lowdensity lipoprotein ELISA kit
E01O0341 has been referenced in the below publications：
THE VAIUATION OF CXCL16 AND ITS CLINICAL SIGNIFICANCE IN CHILDREN WITH PRIMARY NEPHROTIC SYNDROME.
The role of Caveolin-1/NF-kB/HMGBl signal pathway in OX-LDL-induced endothelial injury and the protective effect of probucol on endothelial progenitor cells in hyperlipidemia.
