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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Human oxyntomodulin ELISA kit (E01O0744)

E01O0744 Human oxyntomodulin ELISA kit

The Human oxyntomodulin ELISA kit can be used to identify samples from the human species. Oxyntomodulin can also be called GCG, GLP 1, glucagon, Glucagon like peptide 1, GRPP, GLP-1(7-36), GLP-1(7-37), Oxyntomodulin, OXM, OXY.

Products

Specifications of Human oxyntomodulin ELISA kit

Product Information

Cat. No.

E01O0744

Product Name

Human Human oxyntomodulin ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

25-500 ng/ml

Sensitivity

1.0 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

25 ng/mL

1 vial

STANDARD C (0.5mL)

50 ng/mL

1 vial

STANDARD D (0.5mL)

100 ng/mL

1 vial

STANDARD E (0.5mL)

250 ng/mL

1 vial

STANDARD F (0.5mL)

500 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

OXM ELISA kit uses an anti-OXM antibody and an OXM-HRP conjugate in a competitive enzyme immunoassay method. OXM-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-OXM antibody binding site between OXM from samples and OXM-HRP conjugate, the intensity of the color is inversely proportional to the concentration of OXM. Since the number of sites is limited, as more sites are occupied by OXM from the sample, fewer sites are left to bind OXM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OXM concentration in each sample is interpolated from this standard curve.


Quality Control on Human oxyntomodulin ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between OXM and analogues was observed.


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Summary of the Assay Procedure for Human oxyntomodulin ELISA kit

Summary of the Assay Procedure for Human oxyntomodulin ELISA kit

Citations of Human oxyntomodulin ELISA kit

E01O0744 has been referenced in the below publications:

Specificity and sensitivity of commercially available assays for glucagon and oxyntomodulin measurement in humans.

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