E01O0744 Human oxyntomodulin ELISA kit
The Human oxyntomodulin ELISA kit can be used to identify samples from the human species. Oxyntomodulin can also be called GCG, GLP 1, glucagon, Glucagon like peptide 1, GRPP, GLP-1(7-36), GLP-1(7-37), Oxyntomodulin, OXM, OXY.
Product Information | |
Cat. No. | E01O0744 |
Product Name | Human Human oxyntomodulin ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 25-500 ng/ml |
Sensitivity | 1.0 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 25 ng/mL | 1 vial |
STANDARD C (0.5mL) | 50 ng/mL | 1 vial |
STANDARD D (0.5mL) | 100 ng/mL | 1 vial |
STANDARD E (0.5mL) | 250 ng/mL | 1 vial |
STANDARD F (0.5mL) | 500 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
OXM ELISA kit uses an anti-OXM antibody and an OXM-HRP conjugate in a competitive enzyme immunoassay method. OXM-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-OXM antibody binding site between OXM from samples and OXM-HRP conjugate, the intensity of the color is inversely proportional to the concentration of OXM. Since the number of sites is limited, as more sites are occupied by OXM from the sample, fewer sites are left to bind OXM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OXM concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between OXM and analogues was observed. | |
E01O0744 has been referenced in the below publications：
Specificity and sensitivity of commercially available assays for glucagon and oxyntomodulin measurement in humans.
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