Human Palate, lung, and nasal epithelium carcinoma associated protein ELISA kit (E01P0183)

BlueGene Biotech Human Palate, lung, and nasal epithelium carcinoma associated protein ELISA kit (E01P0183)

E01P0183 Human Palate, lung, and nasal epithelium carcinoma associated protein ELISA kit

The Human Palate, lung, and nasal epithelium carcinoma associated protein ELISA kit can be used to identify samples from the human species. Palate, lung, and nasal epithelium carcinoma associated protein can also be called BPIFA1, SPURT, NASG, LUNX, SPLUNC1, Secretory Protein In Upper Respiratory Tracts, BPI Fold Containing Family A, Member 1, Nasopharyngeal carcinoma-related.

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Specifications of Human Palate, lung, and nasal epithelium carcinoma associated protein ELISA kit

Product Information
Cat. No.	E01P0183
Product Name	Human Palate, lung, and nasal epithelium carcinoma associated protein ELISA kit
Species	Human
Product Size	48 Tests / 96 Tests
Concentration	5.0-100 ng/ml
Sensitivity	1.0 ng/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	5.0 ng/mL	1 vial
STANDARD C (0.5mL)	10 ng/mL	1 vial
STANDARD D (0.5mL)	25 ng/mL	1 vial
STANDARD E (0.5mL)	50 ng/mL	1 vial
STANDARD F (0.5mL)	100 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

PLUNC ELISA kit uses an anti-PLUNC antibody and an PLUNC-HRP conjugate in a competitive enzyme immunoassay method. PLUNC-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-PLUNC antibody binding site between PLUNC from samples and PLUNC-HRP conjugate, the intensity of the color is inversely proportional to the concentration of PLUNC. Since the number of sites is limited, as more sites are occupied by PLUNC from the sample, fewer sites are left to bind PLUNC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PLUNC concentration in each sample is interpolated from this standard curve.

Quality Control on Human Palate, lung, and nasal epithelium carcinoma associated protein ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between PLUNC and analogues was observed.

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Summary of the Assay Procedure for Human Palate, lung, and nasal epithelium carcinoma associated protein ELISA kit

Citations of Human Palate, lung, and nasal epithelium carcinoma associated protein ELISA kit

E01P0183 has been referenced in the below publications：

To investigate the value of CK, CK7, PLUNC in the diagnosis and treatment of Meningeal Carcinomatosis.