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BlueGene Biotech Human Peroxiredoxin 2 ELISA kit (E01P0750)

E01P0750 Human Peroxiredoxin 2 ELISA kit

The Human Peroxiredoxin 2 ELISA kit can be used to identify samples from the human species. Peroxiredoxin 2 can also be called PRP, NKEFB, PRXII, TDPX1, TSA, Thioredoxin-Dependent Peroxide Reductase 1, Thiol-Specific Antioxidant 1, Natural Killer-Enhancing Factor B, Thioredoxin Peroxidase 1, Torin.

Products

Specifications of Human Peroxiredoxin 2 ELISA kit

Product Information

Cat. No.

E01P0750

Product Name

Human Peroxiredoxin 2 ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

2.5-50ng/mL

Sensitivity

0.1 ng/ml

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

2.5 ng/mL

1 vial

STANDARD C (0.5mL)

5.0 ng/mL

1 vial

STANDARD D (0.5mL)

10 ng/mL

1 vial

STANDARD E (0.5mL)

25 ng/mL

1 vial

STANDARD F (0.5mL)

50 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

PRDX2 ELISA kit uses an anti-PRDX2 antibody and an PRDX2-HRP conjugate in a competitive enzyme immunoassay method. PRDX2-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-PRDX2 antibody binding site between PRDX2 from samples and PRDX2-HRP conjugate, the intensity of the color is inversely proportional to the concentration of PRDX2. Since the number of sites is limited, as more sites are occupied by PRDX2 from the sample, fewer sites are left to bind PRDX2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PRDX2 concentration in each sample is interpolated from this standard curve.


Quality Control on Human Peroxiredoxin 2 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between PRDX2 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Peroxiredoxin 2 ELISA kit

Summary of the Assay Procedure for Human Peroxiredoxin 2 ELISA kit

Citations of Human Peroxiredoxin 2 ELISA kit

E01P0750 has been referenced in the below publications:

Proteomics and Peptidomics Analysis of Lung Adenocarcinoma Malignant Pleural Effusion.

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