Human Phosphatidylinositol 4 Kinase Catalytic Alpha ELISA kit (E01P0660)

BlueGene Biotech Human Phosphatidylinositol 4 Kinase Catalytic Alpha ELISA kit (E01P0660)

E01P0660 Human Phosphatidylinositol 4 Kinase Catalytic Alpha ELISA kit

The Human Phosphatidylinositol 4 Kinase Catalytic Alpha ELISA kit can be used to identify samples from the human species. Phosphatidylinositol 4 Kinase Catalytic Alpha can also be called PI4Ka, PI4KA, PI4K-ALPHA, PIK4CA, pi4K230, PMGYCHA, phosphatidylinositol 4-kinase alpha, SPG84, GIDID2, PI4-kinase alpha, PI4K-alpha, PtdIns-4-kinase alpha, PI4-kinase alpha, PI4K-alpha, PtdIns-4-kinase alpha.

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Specifications of Human Phosphatidylinositol 4 Kinase Catalytic Alpha ELISA kit

Product Information
Cat. No.	E01P0660
Product Name	Human Phosphatidylinositol 4 Kinase Catalytic Alpha ELISA kit
Species	Human
Product Size	48 Tests / 96 Tests
Concentration	1.0-25 ng/ml
Sensitivity	0.1 ng/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	1.0 ng/mL	1 vial
STANDARD C (0.5mL)	2.5 ng/mL	1 vial
STANDARD D (0.5mL)	5.0 ng/mL	1 vial
STANDARD E (0.5mL)	10 ng/mL	1 vial
STANDARD F (0.5mL)	25 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

PI4Ka ELISA kit uses an anti-PI4Ka antibody and an PI4Ka-HRP conjugate in a competitive enzyme immunoassay method. PI4Ka-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-PI4Ka antibody binding site between PI4Ka from samples and PI4Ka-HRP conjugate, the intensity of the color is inversely proportional to the concentration of PI4Ka. Since the number of sites is limited, as more sites are occupied by PI4Ka from the sample, fewer sites are left to bind PI4Ka-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PI4Ka concentration in each sample is interpolated from this standard curve.

Quality Control on Human Phosphatidylinositol 4 Kinase Catalytic Alpha ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between PI4Ka and analogues was observed.

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Summary of the Assay Procedure for Human Phosphatidylinositol 4 Kinase Catalytic Alpha ELISA kit

Citations of Human Phosphatidylinositol 4 Kinase Catalytic Alpha ELISA kit

E01P0660 has been referenced in the below publications：

Correlation between expression of PI4KA in serum and HVB DNA load in patients with chronic hepatitis B.