En
Email
  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Human Procollagen Type Ⅲ N Terminal Propeptide ELISA kit (E01P0029)

E01P0029 Human Procollagen Type Ⅲ N Terminal Propeptide ELISA kit

The Human Procollagen Type Ⅲ N Terminal Propeptide ELISA kit can be used to identify samples from the human species. Procollagen Type Ⅲ N Terminal Propeptide can also be called PⅢNP, P3NP, N-Propeptide Of Type III Procollagen, Procollagen III Amino Terminal Propeptide.

Products

Specifications of Human Procollagen Type Ⅲ N Terminal Propeptide ELISA kit

Product Information

Cat. No.

E01P0029

Product Name

Human Procollagen Type Ⅲ N Terminal Propeptide ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

250-5000 pg/ml

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

250 pg/mL

1 vial

STANDARD C (0.5mL)

500 pg/mL

1 vial

STANDARD D (0.5mL)

1000 pg/mL

1 vial

STANDARD E (0.5mL)

2500 pg/mL

1 vial

STANDARD F (0.5mL)

5000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

PⅢNP ELISA kit uses an anti-PⅢNP antibody and an PⅢNP-HRP conjugate in a competitive enzyme immunoassay method. PⅢNP-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-PⅢNP antibody binding site between PⅢNP from samples and PⅢNP-HRP conjugate, the intensity of the color is inversely proportional to the concentration of PⅢNP. Since the number of sites is limited, as more sites are occupied by PⅢNP from the sample, fewer sites are left to bind PⅢNP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PⅢNP concentration in each sample is interpolated from this standard curve.


Quality Control on Human Procollagen Type Ⅲ N Terminal Propeptide ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between PⅢNP and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Procollagen Type Ⅲ N Terminal Propeptide ELISA kit

Summary of the Assay Procedure for Human Procollagen Type Ⅲ N Terminal Propeptide ELISA kit

Citations of Human Procollagen Type Ⅲ N Terminal Propeptide ELISA kit

E01P0029 has been referenced in the below publications:

2型糖尿病患者心肌纤维化血清标志物。

Flavonoid-rich Scabiosa comosa inflorescence extract attenuates CCl4-induced hepatic fibrosis by modulating TGF-β-induced Smad3 phosphorylation.

Related Bluegene Biotech Products

Join us and become BlueGene's distributor, you will get a broad range of Life science research products and more than 12 years of experienced technical supports.