E01P0652 Human Prostaglandin F2a ELISA kit
The Human Prostaglandin F2a ELISA kit can be used to identify samples from the human species. Prostaglandin F2a can also be called PGF2A, PGF2-A, PG-F2a.
Specifications of Human Prostaglandin F2a ELISA kit
Product Information | |
Cat. No. | E01P0652 |
Product Name | Human Prostaglandin F2a ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50ng/mL |
Sensitivity | 0.1ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 10 ng/mL | 1 vial |
STANDARD E (0.5mL) | 25 ng/mL | 1 vial |
STANDARD F (0.5mL) | 50 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
PGF2a ELISA kit uses an anti-PGF2a antibody and an PGF2a-HRP conjugate in a competitive enzyme immunoassay method. PGF2a-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-PGF2a antibody binding site between PGF2a from samples and PGF2a-HRP conjugate, the intensity of the color is inversely proportional to the concentration of PGF2a. Since the number of sites is limited, as more sites are occupied by PGF2a from the sample, fewer sites are left to bind PGF2a-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PGF2a concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Prostaglandin F2a ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between PGF2a and analogues was observed. | |
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Summary of the Assay Procedure for Human Prostaglandin F2a ELISA kit
Citations of Human Prostaglandin F2a ELISA kit
E01P0652 has been referenced in the below publications：
Xeno- and Feeder-Free Differentiation of Human iPSCs to Trabecular Meshwork-Like Cells by Recombinant Cytokines.
