E01P0184 Human PutativeHumanin peptide ELISA kit
The Human PutativeHumanin peptide ELISA kit can be used to identify samples from the human species. PutativeHumanin peptide can also be called MT-RNR2, 16S, Humanin polypeptide, Mitochondrially encoded 16S RNA, Mitochondrial DNA, Humanin, RNR2, mitochondrially encoded 16S RNA, MTRNR2, RNR2, mitochondrially encoded 16S RNA, mtDNA, mDNA.
Product Information | |
Cat. No. | E01P0184 |
Product Name | Human PutativeHumanin peptide ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 100-2500 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 100 pg/mL | 1 vial |
STANDARD C (0.5mL) | 250 pg/mL | 1 vial |
STANDARD D (0.5mL) | 500 pg/mL | 1 vial |
STANDARD E (0.5mL) | 1000 pg/mL | 1 vial |
STANDARD F (0.5mL) | 2500 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
MT-RNR2 ELISA kit uses an anti-MT-RNR2 antibody and an MT-RNR2-HRP conjugate in a competitive enzyme immunoassay method. MT-RNR2-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-MT-RNR2 antibody binding site between MT-RNR2 from samples and MT-RNR2-HRP conjugate, the intensity of the color is inversely proportional to the concentration of MT-RNR2. Since the number of sites is limited, as more sites are occupied by MT-RNR2 from the sample, fewer sites are left to bind MT-RNR2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MT-RNR2 concentration in each sample is interpolated from this standard curve.MT-RNR2. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between MT-RNR2 and analogues was observed. | |
E01P0184 has been referenced in the below publications：
High-dose Humanin analogue applied during ischemia exerts cardioprotection against ischemia/reperfusion injury by reducing mitochondrial dysfunction.
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