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BlueGene Biotech Human Pyruvate Dehydrogenase Kinase Isozyme 4 ELISA kit (E01P0080)

E01P0080 Human Pyruvate Dehydrogenase Kinase Isozyme 4 ELISA kit

The Human Pyruvate Dehydrogenase Kinase Isozyme 4 ELISA kit can be used to identify samples from the human species. Pyruvate Dehydrogenase Kinase Isozyme 4 can also be called kinase isozyme 4, mitochondrial, Pyruvate Dehydrogenase Kinase Isozyme 4.

Products

Specifications of Human Pyruvate Dehydrogenase Kinase Isozyme 4 ELISA kit

Product Information

Cat. No.

E01P0080

Product Name

Human Pyruvate Dehydrogenase Kinase Isozyme 4 ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

2.5-50ng/mL

Sensitivity

0.1 ng/ml

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

2.5 ng/mL

1 vial

STANDARD C (0.5mL)

5.0 ng/mL

1 vial

STANDARD D (0.5mL)

10 ng/mL

1 vial

STANDARD E (0.5mL)

25 ng/mL

1 vial

STANDARD F (0.5mL)

50 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

PDK4 ELISA kit uses an anti-PDK4 antibody and an PDK4-HRP conjugate in a competitive enzyme immunoassay method. PDK4-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-PDK4 antibody binding site between PDK4 from samples and PDK4-HRP conjugate, the intensity of the color is inversely proportional to the concentration of PDK4. Since the number of sites is limited, as more sites are occupied by PDK4 from the sample, fewer sites are left to bind PDK4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PDK4 concentration in each sample is interpolated from this standard curve.


Quality Control on Human Pyruvate Dehydrogenase Kinase Isozyme 4 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between PDK4 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Pyruvate Dehydrogenase Kinase Isozyme 4 ELISA kit

Summary of the Assay Procedure for Human Pyruvate Dehydrogenase Kinase Isozyme 4 ELISA kit

Citations of Human Pyruvate Dehydrogenase Kinase Isozyme 4 ELISA kit

E01P0080 has been referenced in the below publications:

Regulation of the PI3K/AKT Pathway and Fuel Utilization During Primate Torpor in the Gray Mouse Lemur, Microcebus murinus.

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