E01R0022 Human Renin ELISA kit
The Human Renin ELISA kit can be used to identify samples from the human species. Renin can also be called Renin, REN, HNFJ2, renin, ADTKD4, RTD, Angiotensinogenase.
Specifications of Human Renin ELISA kit
Product Information | |
Cat. No. | E01R0022 |
Product Name | Human Renin ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
Renin ELISA kit uses an anti-Renin antibody and a Renin-HRP conjugate in a competitive enzyme immunoassay method. Renin-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-Renin antibody binding site between Renin from samples and Renin-HRP conjugate, the intensity of the color is inversely proportional to the concentration of Renin. Since the number of sites is limited, as more sites are occupied by Renin from the sample, fewer sites are left to bind Renin-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Renin concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Renin ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between Renin and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Human Renin ELISA kit
Citations of Human Renin ELISA kit
E01R0022 has been referenced in the below publications：
Changes of ET-1 and NO in the maintenance hemodialysis complicated with intradialytic hypertension.
Effects of hemodialysis on the cardiovascular function in patients with intradialytic hypertension and the related mechanism.
Changes of the Renin-angiotensin-aldosterone Sysetem During the Perioperative Period in Patients with Mitral Valve Replacement its Clinical Significance.
