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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Human Retinol Binding Protein ELISA kit (E01R0006)

E01R0006 Human Retinol Binding Protein ELISA kit

Human Retinol Binding Protein ELISA kit can be used to identify samples from the human species. Retinol Binding Protein can also be called cellular retinol binding protein 1.

Products

Specifications of Human Retinol Binding Protein ELISA kit

Product Information

Cat. No.

E01R0006

Product Name

Human Retinol Binding Protein ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

25-500mg/L

Sensitivity

1.0mg/L

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 mg/L

1 vial

STANDARD B (0.5mL)

25 mg/L

1 vial

STANDARD C (0.5mL)

50 mg/L

1 vial

STANDARD D (0.5mL)

100 mg/L

1 vial

STANDARD E (0.5mL)

250 mg/L

1 vial

STANDARD F (0.5mL)

500 mg/L

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

RBP ELISA kit uses an anti-RBP antibody and an RBP-HRP conjugate in a competitive enzyme immunoassay method. RBP-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-RBP antibody binding site between RBP from samples and RBP-HRP conjugate, the intensity of the color is inversely proportional to the concentration of RBP. Since the number of sites is limited, as more sites are occupied by RBP from the sample, fewer sites are left to bind RBP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RBP concentration in each sample is interpolated from this standard curve.


Quality Control on Human Retinol Binding Protein ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between RBP and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Retinol Binding Protein ELISA kit

Summary of the Assay Procedure for Human Retinol Binding Protein ELISA kit

Citations of Human Retinol Binding Protein ELISA kit

E01R0006 has been referenced in the below publications:

尿泛素-核糖体结合蛋白52、尿微量清蛋白、尿视黄醇结合蛋白联检在早期糖尿病肾损伤诊断中的价值.

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