E01R0006 Human Retinol Binding Protein ELISA kit
Human Retinol Binding Protein ELISA kit can be used to identify samples from the human species. Retinol Binding Protein can also be called cellular retinol binding protein 1.
Specifications of Human Retinol Binding Protein ELISA kit
Product Information | |
Cat. No. | E01R0006 |
Product Name | Human Retinol Binding Protein ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 25-500mg/L |
Sensitivity | 1.0mg/L |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 mg/L | 1 vial |
STANDARD B (0.5mL) | 25 mg/L | 1 vial |
STANDARD C (0.5mL) | 50 mg/L | 1 vial |
STANDARD D (0.5mL) | 100 mg/L | 1 vial |
STANDARD E (0.5mL) | 250 mg/L | 1 vial |
STANDARD F (0.5mL) | 500 mg/L | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
RBP ELISA kit uses an anti-RBP antibody and an RBP-HRP conjugate in a competitive enzyme immunoassay method. RBP-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-RBP antibody binding site between RBP from samples and RBP-HRP conjugate, the intensity of the color is inversely proportional to the concentration of RBP. Since the number of sites is limited, as more sites are occupied by RBP from the sample, fewer sites are left to bind RBP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RBP concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Retinol Binding Protein ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between RBP and analogues was observed. | |
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Summary of the Assay Procedure for Human Retinol Binding Protein ELISA kit
Citations of Human Retinol Binding Protein ELISA kit
E01R0006 has been referenced in the below publications：
尿泛素-核糖体结合蛋白52、尿微量清蛋白、尿视黄醇结合蛋白联检在早期糖尿病肾损伤诊断中的价值.
