E01R0353 Human RhoA Protein ELISA kit
The Human RhoA Protein ELISA kit can be used to identify samples from the human species. RhoA Protein can also be called RhoA, ARH12, ARHA, RHO12, RHOH12, ras homolog family member A, EDFAOB.
Specifications of Human RhoA Protein ELISA kit
Product Information | |
Cat. No. | E01R0353 |
Product Name | Human RhoA Protein ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25 ng/mL |
Sensitivity | 0.1 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD D (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD E (0.5mL) | 10 ng/mL | 1 vial |
STANDARD F (0.5mL) | 25 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
RhoA ELISA kit uses an anti-RhoA antibody and an RhoA-HRP conjugate in a competitive enzyme immunoassay method. RhoA-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-RhoA antibody binding site between RhoA from samples and RhoA-HRP conjugate, the intensity of the color is inversely proportional to the concentration of RhoA. Since the number of sites is limited, as more sites are occupied by RhoA from the sample, fewer sites are left to bind RhoA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RhoA concentration in each sample is interpolated from this standard curve. |
Quality Control on Human RhoA Protein ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between RhoA and analogues was observed. | |
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Summary of the Assay Procedure for Human RhoA Protein ELISA kit
Citations of Human RhoA Protein ELISA kit
E01R0353 has been referenced in the below publications：
The relationship between RhoA and type 2 diabetes mellitus and its angiopathy.
