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BlueGene Biotech Human S100 Calcium Binding Protein A8 ELISA kit (E01S0038)

E01S0038 Human S100 Calcium Binding Protein A8 ELISA kit

The Human S100 Calcium Binding Protein A8 ELISA kit can be used to identify samples from the human species. S100 Calcium Binding Protein A8 can also be called S100A8, 60B8AG, CAGA, CFAG, CGLA, CP-10, L1Ag, MA387, MIF, MRP8, NIF, p8, P8, S100-A8, Calgranulin-A, calgranulin A, Calprotectin L1L subunit, Chemotactic cytokine CP-10, Cystic fibrosis antigen, L1Ag, Leukocyte L1 complex light chain, MA387, MIF, Migration inhibitory factor-related protein 8, MRP-8, Myeloid-related protein 8, Neutrophil cytosolic 7 kDa protein, Pro-inflammatory S100 cytokine, Protein S100-A8, S100 calcium binding protein A8, S100 calcium binding protein A8, S100 calcium-binding protein A8, S100A8/S100A9 complex, included, Urinary stone protein band A.


Products

Specifications of Human S100 Calcium Binding Protein A8 ELISA kit

Product Information

Cat. No.

E01S0038

Product Name

Human S100 Calcium Binding Protein A8 ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

250-5000 pg/ml

Sensitivity

1.0 pg/ml

Principal

competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/ml

1 vial

STANDARD B (0.5mL)

250 pg/ml

1 vial

STANDARD C (0.5mL)

500 pg/ml

1 vial

STANDARD D (0.5mL)

1000 pg/ml

1 vial

STANDARD E (0.5mL)

2500 pg/ml

1 vial

STANDARD F (0.5mL)

5000 pg/ml

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

S100A8 ELISA kit uses an anti-S100A8 antibody and an S100A8-HRP conjugate in a competitive enzyme immunoassay method. S100A8-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-S100A8 antibody binding site between S100A8 from samples and S100A8-HRP conjugate, the intensity of the color is inversely proportional to the concentration of S100A8. Since the number of sites is limited, as more sites are occupied by S100A8 from the sample, fewer sites are left to bind S100A8-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The S100A8 concentration in each sample is interpolated from this standard curve.


Quality Control on Human S100 Calcium Binding Protein A8 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between S100A8 and analogues was observed.


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Summary of the Assay Procedure for Human S100 Calcium Binding Protein A8 ELISA kit

Summary of the Assay Procedure for Human S100 Calcium Binding Protein A8 ELISA kit

Citations of Human S100 Calcium Binding Protein A8 ELISA kit

E01S0038 has been referenced in the below publications:

Identification of S100A9 as Biomarker of Responsiveness to the Methotrexate/ Etanercept Combination in Rheumatoid Arthritis Using a Proteomic Approach.

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