Human S100 Calcium Binding Protein B ELISA kit (E01S0042)

Specifications of Human S100 Calcium Binding Protein B ELISA kit

Product Information
Cat. No.	E01S0042
Product Name	Human S100 Calcium Binding Protein B ELISA kit
Species	Human
Product Size	48 Tests / 96 Tests
Concentration	50-1000 pg/ml
Sensitivity	1.0 pg/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 pg/mL	1 vial
STANDARD B (0.5mL)	50 pg/mL	1 vial
STANDARD C (0.5mL)	100 pg/mL	1 vial
STANDARD D (0.5mL)	250 pg/mL	1 vial
STANDARD E (0.5mL)	500 pg/mL	1 vial
STANDARD F (0.5mL)	1000 pg/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

S100B ELISA kit uses an anti-S100B antibody and an S100B-HRP conjugate in a competitive enzyme immunoassay method. S100B-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-S100B antibody binding site between S100B from samples and S100B-HRP conjugate, the intensity of the color is inversely proportional to the concentration of S100B. Since the number of sites is limited, as more sites are occupied by S100B from the sample, fewer sites are left to bind S100B-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The S100B concentration in each sample is interpolated from this standard curve.

Quality Control on Human S100 Calcium Binding Protein B ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between S100B and analogues was observed.

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Summary of the Assay Procedure for Human S100 Calcium Binding Protein B ELISA kit

Citations of Human S100 Calcium Binding Protein B ELISA kit

E01S0042 has been referenced in the below publications：

Correlation between expressions of S100B and NSE in serum and cerebrospinal fluid and viral encephalitis in HFMD children.

Study of S100B and NSE in Cerebrospinal Fluid and Serum of patients with Viral Encephalitis caused by Hand Foot and Mouth Disease.

Serum levels of macrophage migration inhibitory factor and S-100B proteins in patients with acute cerebral infarction and their correlation with clinical data.

Correlation among serum S100B protein,NSE and neonatal hypoglycemic brain damage.

Relationship between serum S100B protein, IL-6 and white matter damage in preterm infants.

Effects of lower level controlled hypotension on postoperative cognitive dysfunction in patients affer spinal surgery.

Influence of butyiphthalide on postoperative cognitive dysfunction and Sl00β in elderly patients with laparoscopic cholecystectomy.

The relationship between serum and cerebrospinal fluid S100B concentration and electroencephalograph abnormalities in patients with sepsis encephalopathy.

The relationship between serum SlOOB, neuron-specific enolase and postoperative cognitive dysfunction in the elderly.

The Comparison between sevoflurane and propofol with effect on Cerebral Protection during cardiopulmonary bypass.

Effect of non-cerebral trauma on the value of serum S100B.

Acute hypervolemic hemodilution improves cognitive function and reduces plasma S100β in elderly lung cancer patients following radical surgery.

Curative effect of hypertonic sodium chloridehydroxyethyl starch 40 injection in the treatment of traumatic brain injury combined with hemorrhagic shock and its brain protective mechanism.

Multicenter prospective clinical study to evaluate children short-term neurodevelopmental outcome in congenital heart disease (children NEUROHEART):study protocol.

Serological Characteristics and Cranial MRI Findings in Children Patients with Handfoot-and-mouth Disease Complicated with Encephalitis.