Human Second mitochondrial activator of caspase ELISA kit (E01S0013)

BlueGene Biotech Human Second mitochondrial activator of caspase ELISA kit (E01S0013)

E01S0013 Human Second mitochondrial activator of caspase ELISA kit

The Human Second mitochondrial activator of caspase ELISA kit can be used to identify samples from the bovine species. Second mitochondrial activator of caspase can also be called Smac, DIABLO.

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Specifications of Human Second mitochondrial activator of caspase ELISA kit

Product Information
Cat. No.	E01S0013
Product Name	Human Second mitochondrial activator of caspase ELISA kit
Species	Human
Product Size	48 Tests / 96 Tests
Concentration	5.0-100 ng/mL
Sensitivity	1.0 ng/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	5.0 ng/mL	1 vial
STANDARD C (0.5mL)	10 ng/mL	1 vial
STANDARD D (0.5mL)	25 ng/mL	1 vial
STANDARD E (0.5mL)	50 ng/mL	1 vial
STANDARD F (0.5mL)	100 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

Smac/DIABLO ELISA kit uses an anti-Smac/DIABLO antibody and an Smac/DIABLO-HRP conjugate in a competitive enzyme immunoassay method. Smac/DIABLO-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-Smac/DIABLO antibody binding site between Smac/DIABLO from samples and Smac/DIABLO-HRP conjugate, the intensity of the color is inversely proportional to the concentration of Smac/DIABLO. Since the number of sites is limited, as more sites are occupied by Smac/DIABLO from the sample, fewer sites are left to bind Smac/DIABLO-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Smac/DIABLO concentration in each sample is interpolated from this standard curve.

Quality Control on Human Second mitochondrial activator of caspase ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between Smac/DIABLO and analogues was observed.

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Summary of the Assay Procedure for Human Second mitochondrial activator of caspase ELISA kit

Citations of Human Second mitochondrial activator of caspase ELISA kit

E01S0013 has been referenced in the below publications：

Chronic atrophic gastritis Precancerous lesion of gastric cancer turbidity toxin intrinsic card with Livin Smac/DIABLO and Caspase-3 correlation studies.