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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Human Second mitochondrial activator of caspase ELISA kit (E01S0013)

E01S0013 Human Second mitochondrial activator of caspase ELISA kit

The Human Second mitochondrial activator of caspase ELISA kit can be used to identify samples from the bovine species. Second mitochondrial activator of caspase can also be called Smac, DIABLO.

Products

Specifications of Human Second mitochondrial activator of caspase ELISA kit

Product Information

Cat. No.

E01S0013

Product Name

Human Second mitochondrial activator of caspase ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

5.0-100 ng/mL

Sensitivity

1.0 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

5.0 ng/mL

1 vial

STANDARD C (0.5mL)

10 ng/mL

1 vial

STANDARD D (0.5mL)

25 ng/mL

1 vial

STANDARD E (0.5mL)

50 ng/mL

1 vial

STANDARD F (0.5mL)

100 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

Smac/DIABLO ELISA kit uses an anti-Smac/DIABLO antibody and an Smac/DIABLO-HRP conjugate in a competitive enzyme immunoassay method. Smac/DIABLO-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-Smac/DIABLO antibody binding site between Smac/DIABLO from samples and Smac/DIABLO-HRP conjugate, the intensity of the color is inversely proportional to the concentration of Smac/DIABLO. Since the number of sites is limited, as more sites are occupied by Smac/DIABLO from the sample, fewer sites are left to bind Smac/DIABLO-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Smac/DIABLO concentration in each sample is interpolated from this standard curve.


Quality Control on Human Second mitochondrial activator of caspase ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between Smac/DIABLO and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Second mitochondrial activator of caspase ELISA kit

Summary of the Assay Procedure for Human Second mitochondrial activator of caspase ELISA kit

Citations of Human Second mitochondrial activator of caspase ELISA kit

E01S0013 has been referenced in the below publications:

Chronic atrophic gastritis Precancerous lesion of gastric cancer turbidity toxin intrinsic card with Livin Smac/DIABLO and Caspase-3 correlation studies.

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