NEGES0015 Human Serum Albumin (HSA) ELISA Kit
This Kit is for the quantitative Measurement of Human Serum Albumin Residues in Protein Purification Process, and End-Product (purified fermentation broth, cell culture supernatant, etc.)
Specifications of Human Serum Albumin (HSA) ELISA Kit
Product Information | |
Cat. NO. | NEGES0015 |
Products Name | Human Serum Albumin (HSA) ELISA Kit |
Species | Human |
Product Size | 96 Tests |
Concentration | 0.625-20ng/mL |
Sensitivity | LOD: 0.15ng/mL; LOQ: 0.625ng/mL |
Principal | Sandwich ELISA |
Sample Volume | 100 ul |
Sample Type | ELISA Kit for the quantitative Measurement of Human Serum Albumin Residues in Protein Purification Process, and End-Product (purified fermentation broth, cell culture supernatant, etc.) |
Assay Time | 3 hours and 15 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
Reagents | Specification | Quantity |
Pre-Coated Microplate (Detachable) | 96 wells | 1 plate (Keep Sealed) |
| Standard (Stock Solution) -10ug/ml | 10ul | 1 vial |
Standard S1 | 20 ng/mL | 1 vial |
Standard S2 | 10 ng/mL | 1 vial |
Standard S3 | 5 ng/mL | 1 vial |
| Standard S4 | 2.5 ng/mL | 1 vial |
| Standard S5 | 1.25 ng/mL | 1 vial |
| Standard S6 | 0.625 ng/mL | 1 vial |
HRP-conjugated antibody(200×) | 80ul | 1 vial |
| Diluent Buffer (10×) | 10ml | 1 vial |
| TMB Substrates | 10ml | 1 vial (Avoid Light) |
Stop Solution | 10ml | 1 vial |
Wash Solution (100×) | 10ml | 1 vial |
Plate Sealer | 4 pieces | |
Instruction Manual | 1 | |
Principle of the Assay |
This ELISA kit is applied to the double antibody sandwich Enzyme Linked Immunosorbent Assay to detect the concentration of HSA in samples. The microtiter plate has been pre-coated with anti-HSA antibody, standards or samples are then added to the microtiter plate wells and HSA if present, will bind to the antibody pre-coated wells under specific conditions. Then wash the plate to remove unbound substances, add the HRP-conjugated antibody, and incubate at room temperature (20-25°C) to form a double-antibody sandwich complex with the coated antibody, HSA, and detection antibody.Then a TMB substrate solution is added to the wells to incubate. TMB substrate solution turns blue after the oxidation of HRP, and finally turns to yellow immediately after adding the stop solution. Color of TMB substrate positively correlated with total HSA bound in the initial steps. The color is measured by spectrophotometrically with wavelength of 450nm. The concentration of HSA in samples is then determined by comparing the O.D. of the samples to the standard curve. |
Quality Control on Human Serum Albumin (HSA) ELISA Kit
Coefficient of Variance | Intra Variation:4-9% | ||
Inter Variation: 5.8-9.7% | |||
Specificity/ Cross-reactivity | Sample | Cross-Reactivity（%） | |
BSA | NA | ||
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Summary of the Assay Procedure for Human Serum Albumin (HSA) ELISA Kit
Citations of Human Serum Albumin (HSA) ELISA Kit
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