E01S0228 Human Serum amyloid A ELISA kit
Human Serum amyloid A ELISA kit is suitable for the detection of samples from human species. Serum amyloid A can also be called PIG4, SAA, SAA2, amyloid A, serum, Amyloid fibril protein AA, Amyloid protein A, Serum amyloid A protein, serum amyloid A-1 protein, serum amyloid A1, TP53I4, Tumor protein p53 inducible protein 4.
Product Information | |
Cat. No. | E01S0228 |
Product Name | Human Serum amyloid A ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25 ng/mL |
Sensitivity | 0.1 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD D (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD E (0.5mL) | 10 ng/mL | 1 vial |
STANDARD F (0.5mL) | 25 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
A competitive enzyme immunoassay approach is used in the SAA ELISA kit along with an anti-SAA antibody and an SAA-HRP conjugate. For one hour, the SAA-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. The intensity of the color is inversely proportional to the SAA concentration since SAA from samples and SAA-HRP conjugate compete for the anti-SAA antibody binding site. Since the number of sites is limited, as more sites are occupied by SAA from the sample, fewer sites are left to bind SAA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SAA concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 92-106% | |
Linearity | Diluent Ratio | Range % |
1:2 | 89-109 | |
1:4 | 88-112 | |
1:8 | 85-111 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between SAA and analogues was observed. | |
E01S0228 has been referenced in the below publications：
The study of the Characteristics of Cognitive Impairment after Acute Mild-to-Moderate Ischemic Stroke and the Role of SAA and Aβ in Cognitive Impairment.
Estimated individual inhaled dose of fine particles and indicators of lung function: A pilot study among Chinese young adults.
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