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  • bovine serum albumin elisa 1

BlueGene Biotech Human Serum amyloid A ELISA kit (E01S0228)

E01S0228 Human Serum amyloid A ELISA kit

Human Serum amyloid A ELISA kit is suitable for the detection of samples from human species. Serum amyloid A can also be called PIG4, SAA, SAA2, amyloid A, serum, Amyloid fibril protein AA, Amyloid protein A, Serum amyloid A protein, serum amyloid A-1 protein, serum amyloid A1, TP53I4, Tumor protein p53 inducible protein 4.

Products

Specifications of Human Serum amyloid A ELISA kit

Product Information

Cat. No.

E01S0228

Product Name

Human Serum amyloid A ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

1.0-25 ng/mL

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

1.0 ng/mL

1 vial

STANDARD C (0.5mL)

2.5 ng/mL

1 vial

STANDARD D (0.5mL)

5.0 ng/mL

1 vial

STANDARD E (0.5mL)

10 ng/mL

1 vial

STANDARD F (0.5mL)

25 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

A competitive enzyme immunoassay approach is used in the SAA ELISA kit along with an anti-SAA antibody and an SAA-HRP conjugate. For one hour, the SAA-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. The intensity of the color is inversely proportional to the SAA concentration since SAA from samples and SAA-HRP conjugate compete for the anti-SAA antibody binding site. Since the number of sites is limited, as more sites are occupied by SAA from the sample, fewer sites are left to bind SAA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SAA concentration in each sample is interpolated from this standard curve.


Quality Control on Human Serum amyloid A ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

92-106%

Linearity

Diluent Ratio

Range %

1:2

89-109

1:4

88-112

1:8

85-111

Specificity/Cross-reactivity

No significant cross-reactivity or interference between SAA and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Serum amyloid A ELISA kit

Summary of the Assay Procedure for Human Serum amyloid A ELISA kit

Citations of Human Serum amyloid A ELISA kit

E01S0228 has been referenced in the below publications:

The study of the Characteristics of Cognitive Impairment after Acute Mild-to-Moderate Ischemic Stroke and the Role of SAA and Aβ in Cognitive Impairment.
Estimated individual inhaled dose of fine particles and indicators of lung function: A pilot study among Chinese young adults.


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