E01S0283 Human Solube receptor for advanced glycation end products ELISA kit
The Human Solube receptor for advanced glycation end products ELISA kit can be used to identify samples from the human species. Solube receptor for advanced glycation end products can also be called SRAGE, sAGER, sAger,sRAGE, sSCARJ1, sadvanced glycosylation end product-specific receptor, sadvanced glycosylation end-product specific receptor, ssRAGE.
Product Information | |
Cat. No. | E01S0283 |
Product Name | Human Solube receptor for advanced glycation end products ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 25-500 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 25 pg/mL | 1 vial |
STANDARD C (0.5mL) | 50 pg/mL | 1 vial |
STANDARD D (0.5mL) | 100 pg/mL | 1 vial |
STANDARD E (0.5mL) | 250 pg/mL | 1 vial |
STANDARD F (0.5mL) | 500 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
SRAGE ELISA kit uses an anti-SRAGE antibody and an SRAGE-HRP conjugate in a competitive enzyme immunoassay method. SRAGE-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-SRAGE antibody binding site between SRAGE from samples and SRAGE-HRP conjugate, the intensity of the color is inversely proportional to the concentration of SRAGE. Since the number of sites is limited, as more sites are occupied by SRAGE from the sample, fewer sites are left to bind SRAGE-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SRAGE concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between SRAGE and analogues was observed. | |
E01S0283 has been referenced in the below publications：
Eplerenone restores 24-h blood pressure circadian rhythm and reduces advanced glycation endproducts in rhesus macaques with spontaneous hypertensive metabolic syndrome.
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