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BlueGene Biotech Human Solube receptor for advanced glycation end products ELISA kit (E01S0283)

E01S0283 Human Solube receptor for advanced glycation end products ELISA kit

The Human Solube receptor for advanced glycation end products ELISA kit can be used to identify samples from the human species. Solube receptor for advanced glycation end products can also be called SRAGE, sAGER, sAger,sRAGE, sSCARJ1, sadvanced glycosylation end product-specific receptor, sadvanced glycosylation end-product specific receptor, ssRAGE.

Products

Specifications of Human Solube receptor for advanced glycation end products ELISA kit

Product Information

Cat. No.

E01S0283

Product Name

Human Solube receptor for advanced glycation end products ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

25-500 pg/ml

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

25 pg/mL

1 vial

STANDARD C (0.5mL)

50 pg/mL

1 vial

STANDARD D (0.5mL)

100 pg/mL

1 vial

STANDARD E (0.5mL)

250 pg/mL

1 vial

STANDARD F (0.5mL)

500 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

SRAGE ELISA kit uses an anti-SRAGE antibody and an SRAGE-HRP conjugate in a competitive enzyme immunoassay method. SRAGE-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-SRAGE antibody binding site between SRAGE from samples and SRAGE-HRP conjugate, the intensity of the color is inversely proportional to the concentration of SRAGE. Since the number of sites is limited, as more sites are occupied by SRAGE from the sample, fewer sites are left to bind SRAGE-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SRAGE concentration in each sample is interpolated from this standard curve. 


Quality Control on Human Solube receptor for advanced glycation end products ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between SRAGE and analogues was observed.


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Summary of the Assay Procedure for Human Solube receptor for advanced glycation end products ELISA kit

Summary of the Assay Procedure for Human Solube receptor for advanced glycation end products ELISA kit

Citations of Human Solube receptor for advanced glycation end products ELISA kit

E01S0283 has been referenced in the below publications:

Eplerenone restores 24-h blood pressure circadian rhythm and reduces advanced glycation endproducts in rhesus macaques with spontaneous hypertensive metabolic syndrome.

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