E01F0103 Human Soluble Fms like tyrosine kinase Receptor 1 ELISA kit
Human Soluble Fms like tyrosine kinase Receptor 1 ELISA kit is suitable for the detection of samples from human species. Soluble Fms like tyrosine kinase Receptor 1 can also be called sFLt 1, sFLT1, sFLT, sFLT-1, sVEGFR-1, sVEGFR1, sfms related tyrosine kinase 1, svascular endothelial growth factor receptor 1, sfms related receptor tyrosine kinase 1.
Product Information | |
Cat. No. | E01F0103 |
Product Name | Human Soluble Fms like tyrosine kinase Receptor 1 ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25 ng/mL |
Sensitivity | 0.1 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD D (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD E (0.5mL) | 10 ng/mL | 1 vial |
STANDARD F (0.5mL) | 25 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
A competitive enzyme immunoassay approach is used in the sFLt 1 ELISA kit along with an anti-sFLt 1 antibody and an sFLt 1-HRP conjugate. For one hour, the sFLt 1-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. The intensity of the color is inversely proportional to the sFLt 1 concentration since sFLt 1 from samples and sFLt 1-HRP conjugate compete for the anti-sFLt 1 antibody binding site. Since the number of sites is limited, as more sites are occupied by sFLt 1 from the sample, fewer sites are left to bind sFLt 1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The sFLt 1 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 91-105% | |
Linearity | Diluent Ratio | Range % |
1:2 | 88-106 | |
1:4 | 87-108 | |
1:8 | 85-110 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between sFLt 1 and analogues was observed. | |
E01F0103 has been referenced in the below publications:
The relationship between circulating tissue transglutaminase, soluble fms-like tyrosine kinase-1, soluble endoglin and vascular endothelial growth factor in pre-eclampsia.
孕中晚期PLGF、Sflt-1、sEng的MoM参考值区间研究。
2-Methoxyestradiol deficiency is strongly related to hypertension in early onset severe pre-eclampsia.
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