En
Email
  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Human soluble OX40 Ligand ELISA kit (E01S0320)

  • Neurobiology
  • Epigenetics and Nuclear Signaling
  • Developmental Biology
  • Developmental Biology

E01S0320 Human soluble OX40 Ligand ELISA kit

The Human soluble OX40 Ligand ELISA kit can be used to identify samples from the human species. soluble OX40 Ligand can also be called sOX4L.

Products

Specifications of Human soluble OX40 Ligand ELISA kit

Product Information

Cat. No.

E01S0320

Product Name

Human soluble OX40 Ligand ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

2.5-50ng/mL

Sensitivity

0.1ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

2.5 ng/mL

1 vial

STANDARD C (0.5mL)

5.0 ng/mL

1 vial

STANDARD D (0.5mL)

10 ng/mL

1 vial

STANDARD E (0.5mL)

25 ng/mL

1 vial

STANDARD F (0.5mL)

50 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

sOX4L ELISA kit uses an anti-sOX4L antibody and an sOX4L-HRP conjugate in a competitive enzyme immunoassay method. sOX4L-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-sOX4L antibody binding site between sOX4L from samples and sOX4L-HRP conjugate, the intensity of the color is inversely proportional to the concentration of sOX4L. Since the number of sites is limited, as more sites are occupied by sOX4L from the sample, fewer sites are left to bind sOX4L-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The sOX4L concentration in each sample is interpolated from this standard curve.


Quality Control on Human soluble OX40 Ligand ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between sOX4L and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human soluble OX40 Ligand ELISA kit

Summary of the Assay Procedure for Human soluble OX40 Ligand ELISA kit

Citations of Human soluble OX40 Ligand ELISA kit

E01S0320 has been referenced in the below publications:

Secretory phospholipase A2 and sOX40L levels and their correlations to severity of coronary lesion in acute coronary syndrome.

Related Bluegene Biotech Products

Join us and become BlueGene's distributor, you will get a broad range of Life science research products and more than 12 years of experienced technical supports.