E01S0320 Human soluble OX40 Ligand ELISA kit
The Human soluble OX40 Ligand ELISA kit can be used to identify samples from the human species. soluble OX40 Ligand can also be called sOX4L.
Specifications of Human soluble OX40 Ligand ELISA kit
Product Information | |
Cat. No. | E01S0320 |
Product Name | Human soluble OX40 Ligand ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50ng/mL |
Sensitivity | 0.1ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 10 ng/mL | 1 vial |
STANDARD E (0.5mL) | 25 ng/mL | 1 vial |
STANDARD F (0.5mL) | 50 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
sOX4L ELISA kit uses an anti-sOX4L antibody and an sOX4L-HRP conjugate in a competitive enzyme immunoassay method. sOX4L-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-sOX4L antibody binding site between sOX4L from samples and sOX4L-HRP conjugate, the intensity of the color is inversely proportional to the concentration of sOX4L. Since the number of sites is limited, as more sites are occupied by sOX4L from the sample, fewer sites are left to bind sOX4L-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The sOX4L concentration in each sample is interpolated from this standard curve. |
Quality Control on Human soluble OX40 Ligand ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between sOX4L and analogues was observed. | |
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Summary of the Assay Procedure for Human soluble OX40 Ligand ELISA kit
Citations of Human soluble OX40 Ligand ELISA kit
E01S0320 has been referenced in the below publications：
Secretory phospholipase A2 and sOX40L levels and their correlations to severity of coronary lesion in acute coronary syndrome.
