Human soluble OX40 Ligand ELISA kit (E01S0320) - Shanghai BlueGene Biotech CO., LTD.

BlueGene Biotech Human soluble OX40 Ligand ELISA kit (E01S0320)

Neurobiology

Epigenetics and Nuclear Signaling

Developmental Biology

E01S0320 Human soluble OX40 Ligand ELISA kit

The Human soluble OX40 Ligand ELISA kit can be used to identify samples from the human species. soluble OX40 Ligand can also be called sOX4L.

Products

Specifications of Human soluble OX40 Ligand ELISA kit

Product Information
Cat. No.	E01S0320
Product Name	Human soluble OX40 Ligand ELISA kit
Species	Human
Product Size	48 Tests / 96 Tests
Concentration	2.5-50ng/mL
Sensitivity	0.1ng/mL
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	2.5 ng/mL	1 vial
STANDARD C (0.5mL)	5.0 ng/mL	1 vial
STANDARD D (0.5mL)	10 ng/mL	1 vial
STANDARD E (0.5mL)	25 ng/mL	1 vial
STANDARD F (0.5mL)	50 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

sOX4L ELISA kit uses an anti-sOX4L antibody and an sOX4L-HRP conjugate in a competitive enzyme immunoassay method. sOX4L-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-sOX4L antibody binding site between sOX4L from samples and sOX4L-HRP conjugate, the intensity of the color is inversely proportional to the concentration of sOX4L. Since the number of sites is limited, as more sites are occupied by sOX4L from the sample, fewer sites are left to bind sOX4L-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The sOX4L concentration in each sample is interpolated from this standard curve.

Quality Control on Human soluble OX40 Ligand ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between sOX4L and analogues was observed.