E01S0012 Human Superoxide Dismutase ELISA kit
The Human Superoxide Dismutase ELISA kit can be used to identify samples from the human species. Superoxide Dismutase can also be called SOD, SOD1, Cu-Zn SOD, Ipo1, IPOA, cytosolic, SOD, Soluble, ALS, ALS1, amyotrophic lateral sclerosis 1, adult, Cu, Zn superoxide dismutase, hSod1, indophenoloxidase A, Superoxide dismutase 1, superoxide dismutase 1, soluble, superoxide dismutase, cystolic.
Product Information | |
Cat. No. | E01S0012 |
Product Name | Human Superoxide Dismutase ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25 ug/ml |
Sensitivity | 0.1 ug/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ug/mL | 1 vial |
STANDARD B (0.5mL) | 1.0 ug/mL | 1 vial |
STANDARD C (0.5mL) | 2.5 ug/mL | 1 vial |
STANDARD D (0.5mL) | 5.0 ug/mL | 1 vial |
STANDARD E (0.5mL) | 10 ug/mL | 1 vial |
STANDARD F (0.5mL) | 25 ug/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
SOD ELISA kit uses an anti-SOD antibody and an SOD-HRP conjugate in a competitive enzyme immunoassay method. SOD-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-SOD antibody binding site between SOD from samples and SOD-HRP conjugate, the intensity of the color is inversely proportional to the concentration of SOD. Since the number of sites is limited, as more sites are occupied by SOD from the sample, fewer sites are left to bind SOD-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SOD concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between SOD and analogues was observed. | |
E01S0012 has been referenced in the below publications：
The Clinical Study on the effect of Kun Fu Kang Capsule on Red Cell Immunology with Chronic Pelvic Inflammatory Disease.
Analysis of Cells Types and Protein of Bronchoalveolar Lavage Fluid of Coal Worker's Pneumoconiosis.
阻塞性睡眠呼吸暂停低通气综合征患者线粒体氧化唱抗氧化功能的变化。
A cell model of nonalcoholic steatohepatitis induced by FFA: establishment and dynamic monitoring.
The Effects of Mouse Nerve Growth Factor on Hypoxic-ischemic Encephalopathy and the Neuroprotective Effect in Neonates.
Antioxidant Blueberry Anthocyanins Induce Vasodilation via PI3K/Akt Signaling Pathway in High-Glucose-Induced Human Umbilical Vein Endothelial Cells.
Related Bluegene Biotech Products
BlueGene Biotech News
