E01S0218 Human Survivin ELISA kit
The Human Survivin ELISA kit can be used to identify samples from the human species. Survivin can also be called Surv, BIRC5, API4, EPR-1, baculoviral IAP repeat containing 5.
Specifications of Human Survivin ELISA kit
Product Information | |
Cat. No. | E01S0218 |
Product Name | Human Survivin ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
Surv ELISA kit uses an anti-Surv antibody and an Surv-HRP conjugate in a competitive enzyme immunoassay method. Surv-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-Surv antibody binding site between Surv from samples and Surv-HRP conjugate, the intensity of the color is inversely proportional to the concentration of Surv. Since the number of sites is limited, as more sites are occupied by Surv from the sample, fewer sites are left to bind Surv-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Surv concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Survivin ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between Surv and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Human Survivin ELISA kit
Citations of Human Survivin ELISA kit
E01S0218 has been referenced in the below publications：
Effects of Gonadotropins on the Expression of VEGF and Survivin in the Mouse Ovaries and the Birth Rate after Vitrification Cryopreserved Transplantation.
