E01T0561 Human Testosterone, Free ELISA kit
The Human Testosterone, Free ELISA kit can be used to identify samples from the human species. Testosterone, Free can also be called FT, Testesteron, Testeronun.
Specifications of Human Testosterone, Free ELISA kit
Product Information | |
Cat. No. | E01T0561 |
Product Name | Human Testosterone, Free ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 25-500 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 25 pg/mL | 1 vial |
STANDARD C (0.5mL) | 50 pg/mL | 1 vial |
STANDARD D (0.5mL) | 100 pg/mL | 1 vial |
STANDARD E (0.5mL) | 250 pg/mL | 1 vial |
STANDARD F (0.5mL) | 500 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
FT ELISA kit uses an anti-FT antibody and an FT-HRP conjugate in a competitive enzyme immunoassay method. FT-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-FT antibody binding site between FT from samples and FT-HRP conjugate, the intensity of the color is inversely proportional to the concentration of FT. Since the number of sites is limited, as more sites are occupied by FT from the sample, fewer sites are left to bind FT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FT concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Testosterone, Free ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between FT and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Human Testosterone, Free ELISA kit
Citations of Human Testosterone, Free ELISA kit
E01T0561 has been referenced in the below publications：
The effects of lycium barbarum juice to serum testosterone and sexual function in adult men.
