E01T0922 Human Thioredoxin reductase ELISA kit
The Human Thioredoxin reductase ELISA kit can be used to identify samples from the human species. Thioredoxin reductase can also be called TR, TrxR, Thioredoxin reductase.
Specifications of Human Thioredoxin reductase ELISA kit
Product Information | |
Cat. No. | E01T0922 |
Product Name | Human Thioredoxin reductase ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25 ng/mL |
Sensitivity | 0.1 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD D (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD E (0.5mL) | 10 ng/mL | 1 vial |
STANDARD F (0.5mL) | 25 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
TrxR ELISA kit uses an anti-TrxR antibody and an TrxR-HRP conjugate in a competitive enzyme immunoassay method. TrxR-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-TrxR antibody binding site between TrxR from samples and TrxR-HRP conjugate, the intensity of the color is inversely proportional to the concentration of TrxR. Since the number of sites is limited, as more sites are occupied by TrxR from the sample, fewer sites are left to bind TrxR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TrxR concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Thioredoxin reductase ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 91-105% | |
Linearity | Diluent Ratio | Range % |
1:2 | 88-106 | |
1:4 | 87-108 | |
1:8 | 85-110 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between TrxR and analogues was observed. | |
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Summary of the Assay Procedure for Human Thioredoxin reductase ELISA kit
Citations of Human Thioredoxin reductase ELISA kit
E01T0922 has been referenced in the below publications:
The role of oxidative stress caused by different feeding manners in the formation of emphysema in rats.
