Human Thymidine phosphorylase ELISA kit (E01T0031) - Shanghai BlueGene Biotech CO., LTD.

BlueGene Biotech Human Thymidine phosphorylase ELISA kit (E01T0031)

E01T0031 Human Thymidine phosphorylase ELISA kit

The Human Thymidine phosphorylase ELISA kit can be used to identify samples from the Human species. Thymidine phosphorylase can also be called TYMP, PDECGF, ECGF1, MNGIE, TP, HPD-ECGF, TdRPase, Platelet-Derived Endothelial Cell Growth Factor, Gliostatin.

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Specifications of Human Thymidine phosphorylase ELISA kit

Product Information
Cat. No.	E01T0031
Product Name	Human Thymidine phosphorylase ELISA kit
Species	Human
Product Size	48 Tests / 96 Tests
Concentration	2.5-50 ng/mL
Sensitivity	0.1ng/mL
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	2.5 ng/mL	1 vial
STANDARD C (0.5mL)	5.0 ng/mL	1 vial
STANDARD D (0.5mL)	10 ng/mL	1 vial
STANDARD E (0.5mL)	25 ng/mL	1 vial
STANDARD F (0.5mL)	50 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

TP ELISA kit uses an anti-TP antibody and an TP-HRP conjugate in a competitive enzyme immunoassay method. TP-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-TP antibody binding site between TP from samples and TP-HRP conjugate, the intensity of the color is inversely proportional to the concentration of TP. Since the number of sites is limited, as more sites are occupied by TP from the sample, fewer sites are left to bind TP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TP concentration in each sample is interpolated from this standard curve.

Quality Control on Human Thymidine phosphorylase ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between TP and analogues was observed.

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Summary of the Assay Procedure for Human Thymidine phosphorylase ELISA kit

Citations of Human Thymidine phosphorylase ELISA kit

E01T0031 has been referenced in the below publications：

Spin probe method of electron paramagnetic resonance spectroscopy - a qualitative test for measuring the evolution of dry eye syndrome under treatment.