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BlueGene Biotech Human Thymosin β 4 ELISA kit (E01T0385)

E01T0385 Human Thymosin β 4 ELISA kit

The Human Thymosin β 4 ELISA kit can be used to identify samples from the human species. Thymosin β 4 can also be called Tβ4, Thymosin beta-4, TMSB4X, FX, PTMB4, TB4X, TMSB4, thymosin beta 4, X-linked, thymosin beta 4 X-linked, Seraspenide, Goralatide.

Products

Specifications of Human Thymosin β 4 ELISA kit

Product Information

Cat. No.

E01T0385

Product Name

Human Thymosin β 4 ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

1.0-25 ng/mL

Sensitivity

0.1 ng/mL

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

1.0 ng/mL

1 vial

STANDARD C (0.5mL)

2.5 ng/mL

1 vial

STANDARD D (0.5mL)

5.0 ng/mL

1 vial

STANDARD E (0.5mL)

10 ng/mL

1 vial

STANDARD F (0.5mL)

25 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

Tβ4 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for Tβ4. Standards or samples are then added to the microtiter plate wells and Tβ4 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of Tβ4 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for Tβ4 are added to each well to “sandwich” the Tβ4 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain Tβ4 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Tβ4 concentration in each sample is interpolated from this standard curve.


Quality Control on Human Thymosin β 4 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

91-105%

Linearity

Diluent Ratio

Range %

1:2

88-106

1:4

87-108

1:8

85-110

Specificity/Cross-reactivity

No significant cross-reactivity or interference between Tβ4 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Thymosin β 4 ELISA kit

Summary of the Assay Procedure for Human Thymosin β 4 ELISA kit

Citations of Human Thymosin β 4 ELISA kit

E01T0385 has been referenced in the below publications:

Depletion of Thymosin β4 Promotes the Proliferation, Migration, and Activation of Human Hepatic Stellate Cells.


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