E01T0385 Human Thymosin β 4 ELISA kit
The Human Thymosin β 4 ELISA kit can be used to identify samples from the human species. Thymosin β 4 can also be called Tβ4, Thymosin beta-4, TMSB4X, FX, PTMB4, TB4X, TMSB4, thymosin beta 4, X-linked, thymosin beta 4 X-linked, Seraspenide, Goralatide.
Product Information | |
Cat. No. | E01T0385 |
Product Name | Human Thymosin β 4 ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25 ng/mL |
Sensitivity | 0.1 ng/mL |
Principal | Sandwich ELISA |
Sample Volume | 50 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 10 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD D (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD E (0.5mL) | 10 ng/mL | 1 vial |
STANDARD F (0.5mL) | 25 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
Tβ4 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for Tβ4. Standards or samples are then added to the microtiter plate wells and Tβ4 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of Tβ4 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for Tβ4 are added to each well to “sandwich” the Tβ4 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain Tβ4 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Tβ4 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 91-105% | |
Linearity | Diluent Ratio | Range % |
1:2 | 88-106 | |
1:4 | 87-108 | |
1:8 | 85-110 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between Tβ4 and analogues was observed. | |
E01T0385 has been referenced in the below publications:
Depletion of Thymosin β4 Promotes the Proliferation, Migration, and Activation of Human Hepatic Stellate Cells.
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