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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Human Thyroid transcription factor 1 ELISA kit (E01T0565)

  • Epigenetics and Nuclear Signaling

E01T0565 Human Thyroid transcription factor 1 ELISA kit

The Human Thyroid transcription factor 1 ELISA kit can be used to identify samples from the human species. Thyroid transcription factor 1 can also be called TTF 1, NKX2-1, Nkx2-1, AV026640, Nkx2.1, T/EBP, Titf1, Ttf-1, BCH, BHC, NK-2, NKX2A, TEBP, TTF1, NMTC1, NK2 homeobox 1.

Products

Specifications of Human Thyroid transcription factor 1 ELISA kit

Product Information

Cat. No.

E01T0565

Product Name

Human Thyroid transcription factor 1 ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

1.0-25 ng/mL

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

1.0 ng/mL

1 vial

STANDARD C (0.5mL)

2.5 ng/mL

1 vial

STANDARD D (0.5mL)

5.0 ng/mL

1 vial

STANDARD E (0.5mL)

10 ng/mL

1 vial

STANDARD F (0.5mL)

25 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

TTF 1 ELISA kit uses an anti-TTF 1 antibody and an TTF 1-HRP conjugate in a competitive enzyme immunoassay method. TTF 1-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-TTF 1 antibody binding site between TTF 1 from samples and TTF 1-HRP conjugate, the intensity of the color is inversely proportional to the concentration of TTF 1. Since the number of sites is limited, as more sites are occupied by TTF 1 from the sample, fewer sites are left to bind TTF 1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TTF 1 concentration in each sample is interpolated from this standard curve.


Quality Control on Human Thyroid transcription factor 1 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

91-105%

Linearity

Diluent Ratio

Range %

1:2

88-106

1:4

87-108

1:8

85-110

Specificity/Cross-reactivity

No significant cross-reactivity or interference between TTF 1 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Thyroid transcription factor 1 ELISA kit

Summary of the Assay Procedure for Human Thyroid transcription factor 1 ELISA kit

Citations of Human Thyroid transcription factor 1 ELISA kit

E01T0565 has been referenced in the below publications:

The Research of NKX2-1 with the Biological Effect and Anti-cancer Drugs Resistance in Lung Cancer CeⅡs.

Expression and clinical significance of TTF-1 in the serum of lung adenocarcinoma patients.

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