E01T0015 Human Total Apelin ELISA kit
The Human Total Apelin ELISA kit can be used to identify samples from the human species. Total Apelin can also be called APLN, APEL, XNPEP2, apelin.
Specifications of Human Total Apelin ELISA kit
Product Information | |
Cat. No. | E01T0015 |
Product Name | Human TNF Receptor Associated Factor 6 ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 100-2500 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 100 pg/mL | 1 vial |
STANDARD C (0.5mL) | 250 pg/mL | 1 vial |
STANDARD D (0.5mL) | 500 pg/mL | 1 vial |
STANDARD E (0.5mL) | 1000 pg/mL | 1 vial |
STANDARD F (0.5mL) | 2500 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
Apelin ELISA kit uses an anti-Apelin antibody and an Apelin-HRP conjugate in a competitive enzyme immunoassay method. Apelin-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-Apelin antibody binding site between Apelin from samples and Apelin-HRP conjugate, the intensity of the color is inversely proportional to the concentration of Apelin. Since the number of sites is limited, as more sites are occupied by Apelin from the sample, fewer sites are left to bind Apelin-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Apelin concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Total Apelin ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between Apelin and analogues was observed. | |
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Summary of the Assay Procedure for Human Total Apelin ELISA kit
Citations of Human Total Apelin ELISA kit
E01T0015 has been referenced in the below publications：
Research of plasma Apelin level in hypertensive patients with heart failure.
