En
Email
  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Human Transforming Growth Factor β ELISA kit (E01T0058)

E01T0058 Human Transforming Growth Factor β ELISA kit

The Human Transforming Growth Factor β ELISA kit can be used to identify samples from the human species. Transforming Growth Factor β can also be called TGFβ, TGF-β, TGF-beta.

Products

Specifications of Human Transforming Growth Factor β ELISA kit

Product Information

Cat. No.

E01T0058

Product Name

Human Transforming Growth Factor β ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/ml

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

TGFβ ELISA kit uses an anti-TGFβ antibody and an TGFβ-HRP conjugate in a competitive enzyme immunoassay method. TGFβ-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-TGFβ antibody binding site between TGFβ from samples and TGFβ-HRP conjugate, the intensity of the color is inversely proportional to the concentration of TGFβ. Since the number of sites is limited, as more sites are occupied by TGFβ from the sample, fewer sites are left to bind TGFβ-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TGFβ concentration in each sample is interpolated from this standard curve.


Quality Control on Human Transforming Growth Factor β ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between TGFβ and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Transforming Growth Factor β ELISA kit

Summary of the Assay Procedure for Human Transforming Growth Factor β ELISA kit

Citations of Human Transforming Growth Factor β ELISA kit

E01T0058 has been referenced in the below publications:

Infection of Cattle With Cryptosporidium parvum: Mast Cell Accumulation in Small Intestine Mucosa.

To separation of lactic acid bacteria and yeast cultures for the application of cow and newborn calf perinatal care.

Cryptosporidium parvum. Protective Immunity against Cryptosporidiosis with Recombinant Lactobacillus Live Vaccines Expressing cp966 after Oral Immunization.

Effects of complex anionic salt premix on production performance and health stadus of perinatal cows.

蒲公英水提物对断奶仔猪免疫功能和血清抗氧化功能的效应试验。

Related Bluegene Biotech Products

Join us and become BlueGene's distributor, you will get a broad range of Life science research products and more than 12 years of experienced technical supports.