E01T0018 Human Triglyceride ELISA kit
The Human Triglyceride ELISA kit can be used to identify samples from the human species. Triglyceride can also be called TG, TAG, Triacylglycerol, Triacylglyceride.
Specifications of Human Triglyceride ELISA kit
Product Information | |
Cat. No. | E01T0018 |
Product Name | Human Triglyceride ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 ug/ml |
Sensitivity | 1.0 ug/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ug/mL | 1 vial |
STANDARD B (0.5mL) | 50 ug/mL | 1 vial |
STANDARD C (0.5mL) | 100 ug/mL | 1 vial |
STANDARD D (0.5mL) | 250 ug/mL | 1 vial |
STANDARD E (0.5mL) | 500 ug/mL | 1 vial |
STANDARD F (0.5mL) | 1000 ug/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
TG ELISA kit uses an anti-TG antibody and an TG-HRP conjugate in a competitive enzyme immunoassay method. TG-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-TG antibody binding site between TG from samples and TG-HRP conjugate, the intensity of the color is inversely proportional to the concentration of TG. Since the number of sites is limited, as more sites are occupied by TG from the sample, fewer sites are left to bind TG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TG concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Triglyceride ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between TG and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Human Triglyceride ELISA kit
Citations of Human Triglyceride ELISA kit
E01T0018 has been referenced in the below publications：
Dysregulated hepatic bile acids collaboratively promote liver carcinogenesis.
Effects of Noise on The Hearing And Cardiovascular System In Noise-exposed Workers.
Study on the correlation of estrogen level and high-sensitivity C-reactive protein, blood hornocystein, fibrinogen in postmenopausal women with hypertension.
Short-term Prognostic Impact of Hyponatremia on The Patients with Acute ST-elevation Myocardial Infarction.
Analysis of Heart Rate Variability in Patients with Coronary Artery Non-obstructive Luminal Narrowing and Export the Risk Factors.
Effect of different hemodialysis modalities on resistin and nutritional status.
Study on the correlation between serum estrogen level and the serum levels of homocysteine and fibrinogen level in women with coronary heart disease.
Theabrownin from Pu-erh tea attenuates hypercholesterolemia via modulation of gut microbiota and bile acid metabolism.
