E01T0064 Human Tropomyosin isoform 5 ELISA kit
The Human Tropomyosin isoform 5 ELISA kit can be used to identify samples from the human species. Tropomyosin isoform 5 can also be called TM5.
Specifications of Human Tropomyosin isoform 5 ELISA kit
Product Information | |
Cat. No. | E01T0064 |
Product Name | Human Tropomyosin isoform 5 ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25 ng/ml |
Sensitivity | 0.1 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD D (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD E (0.5mL) | 10 ng/mL | 1 vial |
STANDARD F (0.5mL) | 25 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
TM5 ELISA kit uses an anti-TM5 antibody and an TM5-HRP conjugate in a competitive enzyme immunoassay method. TM5-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-TM5 antibody binding site between TM5 from samples and TM5-HRP conjugate, the intensity of the color is inversely proportional to the concentration of TM5. Since the number of sites is limited, as more sites are occupied by TM5 from the sample, fewer sites are left to bind TM5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TM5 concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Tropomyosin isoform 5 ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between TM5 and analogues was observed. | |
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Summary of the Assay Procedure for Human Tropomyosin isoform 5 ELISA kit
Citations of Human Tropomyosin isoform 5 ELISA kit
E01T0064 has been referenced in the below publications：
Human tropomyosin isoform 5 in ulcerative colitis Expression and Clinical Significance.
Expression of hTM5 in ulcerative colitis and its clinical significance.
