E01T0140 Human Tryptophane ELISA kit
The Human Tryptophane ELISA kit can be used to identify samples from the human species. Tryptophane can also be called 2-Amino-3-(1H-indol-3-yl)propanoic acid.
Specifications of Human Tryptophane ELISA kit
Product Information | |
Cat. No. | E01S0013 |
Product Name | Human Tryptophane ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 5.0-100 ng/mL |
Sensitivity | 1.0 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 10 ng/mL | 1 vial |
STANDARD D (0.5mL) | 25 ng/mL | 1 vial |
STANDARD E (0.5mL) | 50 ng/mL | 1 vial |
STANDARD F (0.5mL) | 100 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
Trp ELISA kit uses an anti-Trp antibody and an Trp-HRP conjugate in a competitive enzyme immunoassay method. Trp-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-Trp antibody binding site between Trp from samples and Trp-HRP conjugate, the intensity of the color is inversely proportional to the concentration of Trp. Since the number of sites is limited, as more sites are occupied by Trp from the sample, fewer sites are left to bind Trp-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Trp concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Tryptophane ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between Trp and analogues was observed. | |
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Summary of the Assay Procedure for Human Tryptophane ELISA kit
Citations of Human Tryptophane ELISA kit
E01T0140 has been referenced in the below publications：
IDO decreases glycolysis and glutaminolysis by activating GCN2K, while it increases fatty acid oxidation by activating AhR, thus preserving CD4+ T‑cell survival and proliferation.
Individualized treatment for allergic rhinitis based on key nasal clinical manifestations combined with histamine and leukotriene D4 levels.
Indoleamine 2, 3-dioxygenase increases p53 levels in alloreactive human T cells, and both indoleamine 2, 3-dioxygenase and p53 suppress glucose uptake
