E01U0007 Human Ubiquitin ELISA kit
The Human Ubiquitin ELISA kit can be used to identify samples from the human species. Ubiquitin can also be called UB, Ubiquitin B, UbB, Ubiquitous Immunopoietic Polypeptide, Polyubiquitin B.
Specifications of Human Ubiquitin ELISA kit
Product Information | |
Cat. No. | E01U0007 |
Product Name | Human Ubiquitin ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 250-5000 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/ml | 1 vial |
STANDARD B (0.5mL) | 250 pg/ml | 1 vial |
STANDARD C (0.5mL) | 500 pg/ml | 1 vial |
STANDARD D (0.5mL) | 1000 pg/ml | 1 vial |
STANDARD E (0.5mL) | 2500 pg/ml | 1 vial |
STANDARD F (0.5mL) | 5000 pg/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
UB ELISA kit uses an anti-UB antibody and an UB-HRP conjugate in a competitive enzyme immunoassay method. UB-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-UB antibody binding site between UB from samples and UB-HRP conjugate, the intensity of the color is inversely proportional to the concentration of UB. Since the number of sites is limited, as more sites are occupied by UB from the sample, fewer sites are left to bind UB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The UB concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Ubiquitin ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between UB and analogues was observed. | |
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Summary of the Assay Procedure for Human Ubiquitin ELISA kit
Citations of Human Ubiquitin ELISA kit
E01U0007 has been referenced in the below publications：
Protection effects of free ubiquitin against radiation and its mechanisms Abstr act.
