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  • bovine serum albumin elisa 1

BlueGene Biotech Human Vascular Cell Adhesion Molecule 1 ELISA kit (E01V0002)

E01V0002 Human Vascular Cell Adhesion Molecule 1 ELISA kit

The Human Vascular Cell Adhesion Molecule 1 ELISA kit can be used to identify samples from the human species. Vascular Cell Adhesion Molecule 1 can also be called CD 106, CD106, CD106 Antigen, INCAM 100, L1CAM, Vascular Cell Adhesion Molecule 1, Vascular cell adhesion protein 1, VCAM 1, VCAM-1, INCAM-100, V-CAM 1.

Products

Specifications of Human Vascular Cell Adhesion Molecule 1 ELISA kit

Product Information

Cat. No.

E01V0002

Product Name

Human Vascular Cell Adhesion Molecule 1 ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

5.0-100 ng/mL

Sensitivity

1.0 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

5.0 ng/mL

1 vial

STANDARD C (0.5mL)

10 ng/mL

1 vial

STANDARD D (0.5mL)

25 ng/mL

1 vial

STANDARD E (0.5mL)

50 ng/mL

1 vial

STANDARD F (0.5mL)

100 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

VCAM1 ELISA kit uses an anti-VCAM1 antibody and an VCAM1-HRP conjugate in a competitive enzyme immunoassay method. VCAM1-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-VCAM1 antibody binding site between VCAM1 from samples and VCAM1-HRP conjugate, the intensity of the color is inversely proportional to the concentration of VCAM1. Since the number of sites is limited, as more sites are occupied by VCAM1 from the sample, fewer sites are left to bind VCAM1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The VCAM1 concentration in each sample is interpolated from this standard curve.


Quality Control on Human Vascular Cell Adhesion Molecule 1 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between VCAM1 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Vascular Cell Adhesion Molecule 1 ELISA kit

Summary of the Assay Procedure for Human Vascular Cell Adhesion Molecule 1 ELISA kit

Citations of Human Vascular Cell Adhesion Molecule 1 ELISA kit

E01V0002 has been referenced in the below publications:

Effect of Prunella vulgaris Decoction combined with Nifedipine in the treatment of primary hypertension.

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