Human Vascular Endothelial Growth Factor Receptor 2 ELISA kit (E01V0004)

BlueGene Biotech Human Vascular Endothelial Growth Factor Receptor 2 ELISA kit (E01V0004)

E01V0004 Human Vascular Endothelial Growth Factor Receptor 2 ELISA kit

The Human Vascular Endothelial Growth Factor Receptor 2 ELISA kit can be used to identify samples from the human species. Vascular Endothelial Growth Factor Receptor 2 can also be called KDR, CD309, FLK1, VEGFR, VEGFR2, VEGFR-2, Kinase insert domain receptor, cluster of differentiation 309.

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Specifications of Human Vascular Endothelial Growth Factor Receptor 2 ELISA kit

Product Information
Cat. No.	E01V0004
Product Name	Human Vascular Endothelial Growth Factor Receptor 2 ELISA kit
Species	Human
Product Size	48 Tests / 96 Tests
Concentration	0.5-10 ng/ml
Sensitivity	0.1 ng/ml
Principal	Sandwich ELISA
Sample Volume	50 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	10.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	0.5 ng/mL	1 vial
STANDARD C (0.5mL)	1.0 ng/mL	1 vial
STANDARD D (0.5mL)	2.5 ng/mL	1 vial
STANDARD E (0.5mL)	5.0 ng/mL	1 vial
STANDARD F (0.5mL)	10 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

VEGFR2 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for VEGFR2. Standards or samples are then added to the microtiter plate wells and VEGFR2 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of VEGFR2 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for VEGFR2 are added to each well to “sandwich” the VEGFR2 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain VEGFR2 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The VEGFR2 concentration in each sample is interpolated from this standard curve.

Quality Control on Human Vascular Endothelial Growth Factor Receptor 2 ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between VEGFR2 and analogues was observed.

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Summary of the Assay Procedure for Human Vascular Endothelial Growth Factor Receptor 2 ELISA kit

Citations of Human Vascular Endothelial Growth Factor Receptor 2 ELISA kit

E01V0004 has been referenced in the below publications：

The clinical study on changs of serum vegf pdge and ang-2 before and after microwave ablation in patients with lung cancer.