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BlueGene Biotech Human Vascular Endothelial Growth Factor Receptor 2 ELISA kit (E01V0004)

E01V0004 Human Vascular Endothelial Growth Factor Receptor 2 ELISA kit

The Human Vascular Endothelial Growth Factor Receptor 2 ELISA kit can be used to identify samples from the human species. Vascular Endothelial Growth Factor Receptor 2 can also be called KDR, CD309, FLK1, VEGFR, VEGFR2, VEGFR-2, Kinase insert domain receptor, cluster of differentiation 309.

Products

Specifications of Human Vascular Endothelial Growth Factor Receptor 2 ELISA kit

Product Information

Cat. No.

E01V0004

Product Name

Human Vascular Endothelial Growth Factor Receptor 2 ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

0.5-10 ng/ml

Sensitivity

0.1 ng/ml

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

0.5 ng/mL

1 vial

STANDARD C (0.5mL)

1.0 ng/mL

1 vial

STANDARD D (0.5mL)

2.5 ng/mL

1 vial

STANDARD E (0.5mL)

5.0 ng/mL

1 vial

STANDARD F (0.5mL)

10 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

VEGFR2 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for VEGFR2. Standards or samples are then added to the microtiter plate wells and VEGFR2 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of VEGFR2 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for VEGFR2 are added to each well to “sandwich” the VEGFR2 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain VEGFR2 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The VEGFR2 concentration in each sample is interpolated from this standard curve.


Quality Control on Human Vascular Endothelial Growth Factor Receptor 2 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between VEGFR2 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Vascular Endothelial Growth Factor Receptor 2 ELISA kit

Summary of the Assay Procedure for Human Vascular Endothelial Growth Factor Receptor 2 ELISA kit

Citations of Human Vascular Endothelial Growth Factor Receptor 2 ELISA kit

E01V0004 has been referenced in the below publications:

The clinical study on changs of serum vegf pdge and ang-2 before and after microwave ablation in patients with lung cancer.

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