E01V0048 Human Visceral adipose Tissue Derived Serine Protease Inhibitor ELISA kit
The Human Visceral adipose Tissue Derived Serine Protease Inhibitor ELISA kit can be used to identify samples from the human species. Visceral adipose Tissue Derived Serine Protease Inhibitor can also be called VASPIN, OL 64, OL-64, OL64, Serpin A12, Serpin A12 precursor, Serpina12, Ser A12, serpin family A member 12.
Product Information | |
Cat. No. | E01V0048 |
Product Name | Human Visceral adipose Tissue Derived Serine Protease Inhibitor ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25 ng/ml |
Sensitivity | 0.1 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD D (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD E (0.5mL) | 10 ng/mL | 1 vial |
STANDARD F (0.5mL) | 25 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
VASPIN ELISA kit uses an anti-VASPIN antibody and an VASPIN-HRP conjugate in a competitive enzyme immunoassay method. VASPIN-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-VASPIN antibody binding site between VASPIN from samples and VASPIN-HRP conjugate, the intensity of the color is inversely proportional to the concentration of VASPIN. Since the number of sites is limited, as more sites are occupied by VASPIN from the sample, fewer sites are left to bind VASPIN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The VASPIN concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between VASPIN and analogues was observed. | |
E01V0048 has been referenced in the below publications：
Plasma levels and placental expression of vaspin in pregnant women with diabetes mellitus.
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