E01V0023 Human Vitamin D ELISA kit
The Human Vitamin D ELISA kit can be used to identify samples from the Human species. Vitamin D can also be called Calciferols.
Specifications of Human Vitamin D ELISA kit
Product Information | |
Cat. No. | E01V0023 |
Product Name | Human Vitamin D ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50 ng/mL |
Sensitivity | 0.1ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 10 ng/mL | 1 vial |
STANDARD E (0.5mL) | 25 ng/mL | 1 vial |
STANDARD F (0.5mL) | 50 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
VD ELISA kit uses an anti-VD antibody and an VD-HRP conjugate in a competitive enzyme immunoassay method. VD-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-VD antibody binding site between VD from samples and VD-HRP conjugate, the intensity of the color is inversely proportional to the concentration of VD. Since the number of sites is limited, as more sites are occupied by VD from the sample, fewer sites are left to bind VD-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The VD concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Vitamin D ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between VD and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Human Vitamin D ELISA kit
Citations of Human Vitamin D ELISA kit
E01V0023 has been referenced in the below publications：
Lrp1 in osteoblasts controls osteoclast activity and protects against osteoporosis by limiting PDGF-RANKL signaling.
