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  • bovine serum albumin elisa 1

BlueGene Biotech Human Yorkie homolog ELISA kit (E01Y0002)

E01Y0002 Human Yorkie homolog ELISA kit

The Human Yorkie homolog ELISA kit can be used to identify samples from the human species. Yorkie homolog can also be called YAP1, COB1, YAP, YAP2, YAP65, YKI, Yes associated protein 1, Yap, Yes1 associated transcriptional regulator, YAP-1, Transcriptional coactivator YAP1, Yes-associated protein 1, Protein yorkie homolog, Yes-associated protein YAP65 homolog.

Products

Specifications of Human Yorkie homolog ELISA kit

Product Information

Cat. No.

E01Y0002

Product Name

Human Yorkie homolog ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

250-5000 pg/ml

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

250 pg/mL

1 vial

STANDARD C (0.5mL)

500 pg/mL

1 vial

STANDARD D (0.5mL)

1000 pg/mL

1 vial

STANDARD E (0.5mL)

2500 pg/mL

1 vial

STANDARD F (0.5mL)

5000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

YAP1 ELISA kit uses an anti-YAP1 antibody and an YAP1-HRP conjugate in a competitive enzyme immunoassay method. YAP1-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-YAP1 antibody binding site between YAP1 from samples and YAP1-HRP conjugate, the intensity of the color is inversely proportional to the concentration of YAP1. Since the number of sites is limited, as more sites are occupied by YAP1 from the sample, fewer sites are left to bind YAP1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The YAP1 concentration in each sample is interpolated from this standard curve.


Quality Control on Human Yorkie homolog ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between YAP1 and analogues was observed.


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Summary of the Assay Procedure for Human Yorkie homolog ELISA kit

Summary of the Assay Procedure for Human Yorkie homolog ELISA kit

Citations of Human Yorkie homolog ELISA kit

E01Y0002 has been referenced in the below publications:

Altered cargo proteins of human plasma endothelial cell-derived exosomes in atherosclerotic cerebrovascular disease.

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