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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Mouse 1, 25 Dihydroxyvitamin D3 ELISA kit (E03D0002)

E03D0002 Mouse 1, 25 Dihydroxyvitamin D3 ELISA kit

The Mouse 1, 25 Dihydroxyvitamin D3 ELISA kit can be used to identify samples from the mouse species. 1, 25 Dihydroxyvitamin D3 can also be called 1, 25 (OH)2D3, 1, 25-dihydroxycholecalciferol, 1alpha, 25-dihydroxyvitamin D3, 1, 25-dihydroxyvitamin D3, 1α, 25-(OH)2D3, Calcitriol.

Products

Specifications of Mouse 1, 25 Dihydroxyvitamin D3 ELISA kit

Product Information

Cat. No.

E03D0002

Product Name

Mouse 1, 25 Dihydroxyvitamin D3 ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

50-1000pg/ml

Sensitivity

1.0pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/ml1 vial

STANDARD B (0.5mL)

50 pg/ml

1 vial

STANDARD C (0.5mL)

100 pg/ml

1 vial

STANDARD D (0.5mL)

250 pg/ml

1 vial

STANDARD E (0.5mL)

500 pg/ml

1 vial

STANDARD F (0.5mL)

1000 pg/ml

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

1, 25 (OH)2D3 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-1, 25 (OH)2D3 antibody and an 1, 25 (OH)2D3-HRP conjugate. The assay sample and buffer are incubated together with 1, 25 (OH)2D3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the 1, 25 (OH)2D3 concentration since 1, 25 (OH)2D3 from samples and 1, 25 (OH)2D3-HRP conjugate compete for the anti-1, 25 (OH)2D3 antibody binding site. Since the number of sites is limited, as more sites are occupied by 1, 25 (OH)2D3 from the sample, fewer sites are left to bind 1, 25 (OH)2D3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The 1, 25 (OH)2D3 concentration in each sample is interpolated from this standard curve.


Quality Control on Mouse 1, 25 Dihydroxyvitamin D3 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between 1, 25 (OH)2D3 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Mouse 1, 25 Dihydroxyvitamin D3 ELISA kit

Summary of the Assay Procedure for Mouse 1, 25 Dihydroxyvitamin D3 ELISA kit

Citations of Mouse 1, 25 Dihydroxyvitamin D3 ELISA kit

E03D0002 has been referenced in the below publications:

Oleanolic Acid Exerts Osteoprotective Effects and Modulates Vitamin D Metabolism.

In vitro vitamin K2 and 1α, 25-dihydroxyvitamin D3 combination enhances osteoblasts anabolism of diabetic mice.

Ultraviolet B light attenuates the systemic immune response in central nervous system autoimmunity.


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