E03A0711 Mouse Acetylcholine ELISA kit
The Mouse Acetylcholine ELISA kit can be used to identify samples from the mouse species. Acetylcholine can also be called Acetylcholine,ACh, acetycholine, ACH．
Specifications of Mouse Acetylcholine ELISA kit
Product Information | |
Cat. No. | E03A0711 |
Product Name | Mouse Acetylcholine ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10ng/ml |
Sensitivity | 0.1ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/ml | 1 vial |
STANDARD B (0.5mL) | 0.5 ng/ml | 1 vial |
STANDARD C (0.5mL) | 1.0 ng/ml | 1 vial |
STANDARD D (0.5mL) | 2.5 ng/ml | 1 vial |
STANDARD E (0.5mL) | 5.0ng/ml | 1 vial |
STANDARD F (0.5mL) | 10 ng/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
ACH ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-ACH antibody and a ACH-HRP conjugate. The assay sample and buffer are incubated together with ACH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ACH concentration since ACH from samples and ACH-HRP conjugate compete for the anti-ACH antibody binding site. Since the number of sites is limited, as more sites are occupied by ACH from the sample, fewer sites are left to bind ACH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACH concentration in each sample is interpolated from this standard curve. |
Quality Control on Mouse Acetylcholine ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between ACH and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Mouse Acetylcholine ELISA kit
Citations of Mouse Acetylcholine ELISA kit
E03A0711 has been referenced in the below publications：
Intranasal administration of TAT-haFGF14-154 attenuates disease progression in a mouse model of alzheimer's disease.
Intranasal administration of TAT-haFGF14-154 attenuates disease progression in a mouse model of alzheimer's disease.
Changes of neuronal acetylcholine receptor alpha 7 of peritoneal macrophage in experimental acute pancreatitis treated by Chaiqin Chengqi Decoction.
