E03A0893 Mouse Adipose Triglyceride Lipase ELISA kit
The Mouse Adipose Triglyceride Lipase ELISA kit can be used to identify samples from the mouse species. Adipose Triglyceride Lipase can also be called PNPLA2, 1110001C14Rik, ATGL, PEDF-R, TTS-2.2, TTS2, iPLA2zeta, FP17548, patatin like phospholipase domain containing 2.
Product Information | |
Cat. No. | E03A0893 |
Product Name | Mouse Adipose Triglyceride Lipase ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 10-250 ng/mL |
Sensitivity | 1.0ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 10 ng/mL | 1 vial |
STANDARD C (0.5mL) | 25 ng/mL | 1 vial |
STANDARD D (0.5mL) | 50 ng/mL | 1 vial |
STANDARD E (0.5mL) | 100 ng/mL | 1 vial |
STANDARD F (0.5mL) | 250 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
ATGL ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-ATGL antibody and an ATGL-HRP conjugate. The assay sample and buffer are incubated together with ATGL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATGL concentration since ATGL from samples and ATGL-HRP conjugate compete for the anti-ATGL antibody binding site. Since the number of sites is limited, as more sites are occupied by ATGL from the sample, fewer sites are left to bind ATGL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATGL concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between ATGL and analogues was observed. | |
E03A0893 has been referenced in the below publications：
Effect of common edible oils on lipid metabolism and its mechanism in obese mice induced by a high fat diet.
Effect of medium chain triglycerides on brown adipose tissue in obese mice.
Medium-Chain Triglyceride Activated Brown Adipose Tissue and Induced Reduction of Fat Mass in C57BL/6J Mice Fed High-fat Diet.
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