E03A0388 Mouse Alanine Aminotransferase ELISA kit
The Mouse Alanine Aminotransferase ELISA kit can be used to identify samples from the mouse species. Alanine Aminotransferase can also be called Alanine aminotransferase, AAT1, Alanine aminotransferase 1, ALT1, Glutamic alanine transaminase 1, Glutamic pyruvate transaminase, Glutamic pyruvic transaminase 1, GPT, GPT1, SGPT, ALAT1, ALT.
Product Information | |
Cat. No. | E03A0388 |
Product Name | Mouse Alanine Aminotransferase ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 10-250 U/L |
Sensitivity | 1.0U/L |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 U/L | 1 vial |
STANDARD B (0.5mL) | 10 U/L | 1 vial |
STANDARD C (0.5mL) | 25 U/L | 1 vial |
STANDARD D (0.5mL) | 50 U/L | 1 vial |
STANDARD E (0.5mL) | 100 U/L | 1 vial |
STANDARD F (0.5mL) | 250 U/L | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
ALT ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-ALT antibody and an ALT-HRP conjugate. The assay sample and buffer are incubated together with ALT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ALT concentration since ALT from samples and ALT-HRP conjugate compete for the anti-ALT antibody binding site. Since the number of sites is limited, as more sites are occupied by ALT from the sample, fewer sites are left to bind ALT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ALT concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between ALT and analogues was observed. | |
E03A0388 has been referenced in the below publications：
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