Mouse Aldehyde Dehydrogenase Family 1 Member A3 ELISA kit (E03A0523)

BlueGene Biotech Mouse Aldehyde Dehydrogenase Family 1 Member A3 ELISA kit (E03A0523)

E03A0523 Mouse Aldehyde Dehydrogenase Family 1 Member A3 ELISA kit

The Mouse Aldehyde Dehydrogenase Family 1 Member A3 ELISA kit can be used to identify samples from the mouse species. Aldehyde Dehydrogenase Family 1 Member A3 can also be called ALDH1A3, ALDH1A6, ALDH6, MCOP8, RALDH3, aldehyde dehydrogenase 1 family member A3.

Products

Specifications of Mouse Aldehyde Dehydrogenase Family 1 Member A3 ELISA kit

Product Information
Cat. No.	E03A0523
Product Name	Mouse Aldehyde Dehydrogenase Family 1 Member A3 ELISA kit
Species	Mouse
Product Size	48 Tests / 96 Tests
Concentration	1.0-25ng/ml
Sensitivity	0.1ng/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/ml	1 vial
STANDARD B (0.5mL)	1.0 ng/ml	1 vial
STANDARD C (0.5mL)	2.5 ng/ml	1 vial
STANDARD D (0.5mL)	5.0 ng/ml	1 vial
STANDARD E (0.5mL)	10 ng/ml	1 vial
STANDARD F (0.5mL)	25 ng/ml	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

ALDH1A3 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-ALDH1A3 antibody and an ALDH1A3-HRP conjugate. The assay sample and buffer are incubated together with ALDH1A3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ALDH1A3 concentration since ALDH1A3 from samples and ALDH1A3-HRP conjugate compete for the anti-ALDH1A3 antibody binding site. Since the number of sites is limited, as more sites are occupied by ALDH1A3 from the sample, fewer sites are left to bind ALDH1A3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ALDH1A3 concentration in each sample is interpolated from this standard curve.

Quality Control on Mouse Aldehyde Dehydrogenase Family 1 Member A3 ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between ALDH1A3 and analogues was observed.