E03A0773 Mouse Aldose Reductase ELISA kit
The Mouse Aldose Reductase ELISA kit can be used to identify samples from the mouse species. Aldose Reductase can also be called AR, aldo-keto reductase family 1, member B1 (aldose reductase).
Specifications of Mouse Aldose Reductase ELISA kit
Product Information | |
Cat. No. | E03A0773 |
Product Name | Mouse Aldose Reductase ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50ng/ml |
Sensitivity | 0.1ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/ml | 1 vial |
STANDARD B (0.5mL) | 2.5 ng/ml | 1 vial |
STANDARD C (0.5mL) | 5.0 ng/ml | 1 vial |
STANDARD D (0.5mL) | 10 ng/ml | 1 vial |
STANDARD E (0.5mL) | 25 ng/ml | 1 vial |
STANDARD F (0.5mL) | 50 ng/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
AR ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-AR antibody and an AR-HRP conjugate. The assay sample and buffer are incubated together with AR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AR concentration since AR from samples and AR-HRP conjugate compete for the anti-AR antibody binding site. Since the number of sites is limited, as more sites are occupied by AR from the sample, fewer sites are left to bind AR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AR concentration in each sample is interpolated from this standard curve. |
Quality Control on Mouse Aldose Reductase ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between AR and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Mouse Aldose Reductase ELISA kit
Citations of Mouse Aldose Reductase ELISA kit
E03A0773 has been referenced in the below publications：
Study on Protective Effects of Phlegm medicine Chenpi against Skin Photoaging.
