E03D0004 Mouse Anti Double Stranded DNA ELISA kit
Mouse Anti Double Stranded DNA ELISA kit is suitable for detecting samples from mouse species. Anti Double Stranded DNA can also be called dsDNA-Ab, anti dsDNA.
Specifications of Mouse Anti Double Stranded DNA ELISA kit
Product Information | |
Cat. No. | E03D0004 |
Product Name | Mouse Anti Double Stranded DNA ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 ng/mL |
Sensitivity | 1.0 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 50 ng/mL | 1 vial |
STANDARD C (0.5mL) | 100 ng/mL | 1 vial |
STANDARD D (0.5mL) | 250 ng/mL | 1 vial |
STANDARD E (0.5mL) | 500 ng/mL | 1 vial |
STANDARD F (0.5mL) | 1000 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
anti dsDNA ELISA kit applies the competitive enzyme immunoassay technique utilizing a dsDNA antigen and an anti dsDNA-HRP conjugate. The assay sample and buffer are incubated together with anti dsDNA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the anti dsDNA concentration since anti dsDNA from samples and anti dsDNA-HRP conjugate compete for the dsDNA antigen binding site. Since the number of sites is limited, as more sites are occupied by anti dsDNA from the sample, fewer sites are left to bind anti dsDNA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The anti dsDNA concentration in each sample is interpolated from this standard curve. |
Quality Control on Mouse Anti Double Stranded DNA ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 98-104% | |
Linearity | Diluent Ratio | Range % |
1:2 | 96-103 | |
1:4 | 95-107 | |
1:8 | 91-102 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between anti dsDNA and analogues was observed. | |
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Summary of the Assay Procedure for Mouse Anti Double Stranded DNA ELISA kit
Citations of Mouse Anti Double Stranded DNA ELISA kit
E03D0004 has been referenced in the below publications:
The effect of langchuangqing granule on anti-ds-DNA antibody，IgG and IgA level in MRL/lpr lupus mice.
Toll-like receptor 2-modulating pectin-polymers in alginate-based microcapsules attenuate immune responses and support islet-xenograft survival.
